Functional co-assembly among subunits of cyclic-nucleotide-activated, nonselective cation channels, and across species from nematode to human

J. T. Finn, D. Krautwurst, J. E. Schroeder, Tsung-Yu Chen, R. R. Reed, K. W. Yau

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Cyclic-nucleotide-activated, nonselective cation channels have a central role in sensory transduction. They are most likely tetramers, composed of two subunits (α and β or 1 and 2), with the former, but not the latter, being able to form homomeric cyclic-nucleotide-activated channels. Identified members of this channel family now include, in vertebrates, the rod and cone channels mediating visual transduction and the channel mediating olfactory transduction, each apparently with distinct α- and β-subunits. Homologous channels have also been identified in Drosophila melanogaster and Caenorhabditis elegans. By co-expressing any combination of two α-subunits, or α- and β-subunits, of this channel family in HEK 293 cells, we have found that they can all co-assemble functionally with each other, including those from fly and nematode. This finding suggests that the subunit members so far identified form a remarkably homogeneous and conserved group, functionally and evolutionarily, with no subfamilies yet identified. The ability to cross-assemble allows these subunits to potentially generate a diversity of heteromeric channels, each with properties specifically suited to a particular cellular function.

Original languageEnglish (US)
Pages (from-to)1333-1345
Number of pages13
JournalBiophysical Journal
Volume74
Issue number3
StatePublished - Mar 1998
Externally publishedYes

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Cyclic Nucleotides
Cations
Vertebrate Photoreceptor Cells
Aptitude
HEK293 Cells
Caenorhabditis elegans
Drosophila melanogaster
Diptera
Vertebrates

ASJC Scopus subject areas

  • Biophysics

Cite this

Functional co-assembly among subunits of cyclic-nucleotide-activated, nonselective cation channels, and across species from nematode to human. / Finn, J. T.; Krautwurst, D.; Schroeder, J. E.; Chen, Tsung-Yu; Reed, R. R.; Yau, K. W.

In: Biophysical Journal, Vol. 74, No. 3, 03.1998, p. 1333-1345.

Research output: Contribution to journalArticle

Finn, J. T. ; Krautwurst, D. ; Schroeder, J. E. ; Chen, Tsung-Yu ; Reed, R. R. ; Yau, K. W. / Functional co-assembly among subunits of cyclic-nucleotide-activated, nonselective cation channels, and across species from nematode to human. In: Biophysical Journal. 1998 ; Vol. 74, No. 3. pp. 1333-1345.
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