Abstract
Little is known regarding the timing of immune ontogeny and effector function in fetal humans and nonhuman primates. We studied the organization of lymphocyte and antigen-presenting cell populations in developing lymphoid tissues of rhesus monkey fetuses during the second and third trimesters (65 to 145 days of gestation; term = 165 days). Immunoglobulin-secreting and cytokine-secreting cells were detected at day 80. The thymus, spleen, lymph nodes, and intestinal mucosa were examined for cells expressing CD3, CD5, CD20, CD68, p55, and HLA-DR. In the spleens of 65-day-old fetuses (early second trimester), the overwhelming majority of total lymphocytes were CD5+ CD20+ B-1 cells. The remaining lymphocytes were CD3+ T cells. By day 80, splenic B and T cells were equal in number. Intraepithelial CD3+ CD5- T cells and lamina propria CD20+ CD5+ B cells were present in the intestines of 65-day-old fetuses. By day 80, numerous CD20+ CD5+ B cells were present in the jejunums and colons and early lymphocyte aggregate formation was evident. The spleens of 80- to 145-day-old fetuses contained immunoglobulin M (IgM)-secreting cells, while IgA-, IgG-, interleukin-6-, and gamma interferon-secreting cells were numerous in the spleens and colons. Thus, by the second trimester, the lymphoid tissues of the rhesus monkey fetus have a complete repertoire of properly organized antigen-presenting cells, T cells, and B cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 140-153 |
| Number of pages | 14 |
| Journal | Clinical and Diagnostic Laboratory Immunology |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2003 |
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ASJC Scopus subject areas
- Microbiology (medical)
- Immunology and Allergy
- Clinical Biochemistry
- Immunology
Cite this
Functional and morphological development of lymphoid tissues and immune regulatory and effector function in rhesus monkeys : Cytokine-secreting cells, immunoglobulin-secreting cells, and CD5+ B-1 cells appear early in fetal development. / Makori, Norbert; Tarantal, Alice F; Lü, Fabien X.; Rourke, Tracy; Marthas, Marta; McChesney, Michael B.; Hendrickx, Andrew G; Miller, Chris J.
In: Clinical and Diagnostic Laboratory Immunology, Vol. 10, No. 1, 01.2003, p. 140-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional and morphological development of lymphoid tissues and immune regulatory and effector function in rhesus monkeys
T2 - Cytokine-secreting cells, immunoglobulin-secreting cells, and CD5+ B-1 cells appear early in fetal development
AU - Makori, Norbert
AU - Tarantal, Alice F
AU - Lü, Fabien X.
AU - Rourke, Tracy
AU - Marthas, Marta
AU - McChesney, Michael B.
AU - Hendrickx, Andrew G
AU - Miller, Chris J
PY - 2003/1
Y1 - 2003/1
N2 - Little is known regarding the timing of immune ontogeny and effector function in fetal humans and nonhuman primates. We studied the organization of lymphocyte and antigen-presenting cell populations in developing lymphoid tissues of rhesus monkey fetuses during the second and third trimesters (65 to 145 days of gestation; term = 165 days). Immunoglobulin-secreting and cytokine-secreting cells were detected at day 80. The thymus, spleen, lymph nodes, and intestinal mucosa were examined for cells expressing CD3, CD5, CD20, CD68, p55, and HLA-DR. In the spleens of 65-day-old fetuses (early second trimester), the overwhelming majority of total lymphocytes were CD5+ CD20+ B-1 cells. The remaining lymphocytes were CD3+ T cells. By day 80, splenic B and T cells were equal in number. Intraepithelial CD3+ CD5- T cells and lamina propria CD20+ CD5+ B cells were present in the intestines of 65-day-old fetuses. By day 80, numerous CD20+ CD5+ B cells were present in the jejunums and colons and early lymphocyte aggregate formation was evident. The spleens of 80- to 145-day-old fetuses contained immunoglobulin M (IgM)-secreting cells, while IgA-, IgG-, interleukin-6-, and gamma interferon-secreting cells were numerous in the spleens and colons. Thus, by the second trimester, the lymphoid tissues of the rhesus monkey fetus have a complete repertoire of properly organized antigen-presenting cells, T cells, and B cells.
AB - Little is known regarding the timing of immune ontogeny and effector function in fetal humans and nonhuman primates. We studied the organization of lymphocyte and antigen-presenting cell populations in developing lymphoid tissues of rhesus monkey fetuses during the second and third trimesters (65 to 145 days of gestation; term = 165 days). Immunoglobulin-secreting and cytokine-secreting cells were detected at day 80. The thymus, spleen, lymph nodes, and intestinal mucosa were examined for cells expressing CD3, CD5, CD20, CD68, p55, and HLA-DR. In the spleens of 65-day-old fetuses (early second trimester), the overwhelming majority of total lymphocytes were CD5+ CD20+ B-1 cells. The remaining lymphocytes were CD3+ T cells. By day 80, splenic B and T cells were equal in number. Intraepithelial CD3+ CD5- T cells and lamina propria CD20+ CD5+ B cells were present in the intestines of 65-day-old fetuses. By day 80, numerous CD20+ CD5+ B cells were present in the jejunums and colons and early lymphocyte aggregate formation was evident. The spleens of 80- to 145-day-old fetuses contained immunoglobulin M (IgM)-secreting cells, while IgA-, IgG-, interleukin-6-, and gamma interferon-secreting cells were numerous in the spleens and colons. Thus, by the second trimester, the lymphoid tissues of the rhesus monkey fetus have a complete repertoire of properly organized antigen-presenting cells, T cells, and B cells.
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U2 - 10.1128/CDLI.10.1.140-153.2003
DO - 10.1128/CDLI.10.1.140-153.2003
M3 - Article
VL - 10
SP - 140
EP - 153
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
SN - 1556-6811
IS - 1
ER -