Functional and immunological characterization of SIV envelope glycoprotein produced in genetically engineered mammalian cells

V. Planelles, N. L. Haigwood, Marta Marthas, K. A. Mann, C. Scandella, W. D. Lidster, J. R. Shuster, R. Van Kuyk, P. A. Marx, M. B. Gardner, Paul A Luciw

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Retroviral envelope glycoproteins interact with cell receptors and are targets for antiviral immune responses in infected hosts. Macaque simian immunodeficiency virus (SIV(mac)) is a T-lymphocytopathic lentivirus which causes an AIDS-like disease in rhesus macaques. The envelope gene of SIV(mac) encodes a precursor glycoprotein (gp160) which is cleaved into an external domain (gp130) and a transmembrane domain (gp32). To investigate the functional and immunological properties of the SIV external envelope glycoprotein, we have used genetically engineered mammalian cells to produce recombinant gp130 (rgp130). The rgp130 has the appropriate molecular weight, is glycosylated, and has native conformation as determined by binding to the cell receptor for SIV, the CD4 antigen. Rhesus macaques immunized with purified rgp130 formulated in muramyl dipeptide adjuvant generated high titers of antienvelope antibodies. Antibodies from thse macaques were tested for in vitro virus neutralization; very low or undetectable levels of neutralization were observed. In contrast, neutralizing antibodies were readily detected in sera from goats immunized with rgp130. With respect to cell-mediated immunity, proliferative responses to rgp130 were demonstrated in peripheral blood monocyte cells (PBMC) from macaques immunized with the recombinant glycoprotein as well as in PBMC from SIV-infected animals. These results show that rgp130 is functional and immunogenic; the potential of rgp130 for protective immunization remains to be determined.

Original languageEnglish (US)
Pages (from-to)889-898
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume7
Issue number11
StatePublished - 1991

Fingerprint

Glycoproteins
Macaca
Macaca mulatta
Monocytes
Blood Cells
Acetylmuramyl-Alanyl-Isoglutamine
Simian Immunodeficiency Virus
CD4 Antigens
Lentivirus
Antibodies
Neutralizing Antibodies
Goats
Cellular Immunity
Antiviral Agents
Immunization
Acquired Immunodeficiency Syndrome
Molecular Weight
Viruses
Serum
Genes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Functional and immunological characterization of SIV envelope glycoprotein produced in genetically engineered mammalian cells. / Planelles, V.; Haigwood, N. L.; Marthas, Marta; Mann, K. A.; Scandella, C.; Lidster, W. D.; Shuster, J. R.; Van Kuyk, R.; Marx, P. A.; Gardner, M. B.; Luciw, Paul A.

In: AIDS Research and Human Retroviruses, Vol. 7, No. 11, 1991, p. 889-898.

Research output: Contribution to journalArticle

Planelles, V, Haigwood, NL, Marthas, M, Mann, KA, Scandella, C, Lidster, WD, Shuster, JR, Van Kuyk, R, Marx, PA, Gardner, MB & Luciw, PA 1991, 'Functional and immunological characterization of SIV envelope glycoprotein produced in genetically engineered mammalian cells', AIDS Research and Human Retroviruses, vol. 7, no. 11, pp. 889-898.
Planelles, V. ; Haigwood, N. L. ; Marthas, Marta ; Mann, K. A. ; Scandella, C. ; Lidster, W. D. ; Shuster, J. R. ; Van Kuyk, R. ; Marx, P. A. ; Gardner, M. B. ; Luciw, Paul A. / Functional and immunological characterization of SIV envelope glycoprotein produced in genetically engineered mammalian cells. In: AIDS Research and Human Retroviruses. 1991 ; Vol. 7, No. 11. pp. 889-898.
@article{4d1ba821f6374700803bb3230f3f63fb,
title = "Functional and immunological characterization of SIV envelope glycoprotein produced in genetically engineered mammalian cells",
abstract = "Retroviral envelope glycoproteins interact with cell receptors and are targets for antiviral immune responses in infected hosts. Macaque simian immunodeficiency virus (SIV(mac)) is a T-lymphocytopathic lentivirus which causes an AIDS-like disease in rhesus macaques. The envelope gene of SIV(mac) encodes a precursor glycoprotein (gp160) which is cleaved into an external domain (gp130) and a transmembrane domain (gp32). To investigate the functional and immunological properties of the SIV external envelope glycoprotein, we have used genetically engineered mammalian cells to produce recombinant gp130 (rgp130). The rgp130 has the appropriate molecular weight, is glycosylated, and has native conformation as determined by binding to the cell receptor for SIV, the CD4 antigen. Rhesus macaques immunized with purified rgp130 formulated in muramyl dipeptide adjuvant generated high titers of antienvelope antibodies. Antibodies from thse macaques were tested for in vitro virus neutralization; very low or undetectable levels of neutralization were observed. In contrast, neutralizing antibodies were readily detected in sera from goats immunized with rgp130. With respect to cell-mediated immunity, proliferative responses to rgp130 were demonstrated in peripheral blood monocyte cells (PBMC) from macaques immunized with the recombinant glycoprotein as well as in PBMC from SIV-infected animals. These results show that rgp130 is functional and immunogenic; the potential of rgp130 for protective immunization remains to be determined.",
author = "V. Planelles and Haigwood, {N. L.} and Marta Marthas and Mann, {K. A.} and C. Scandella and Lidster, {W. D.} and Shuster, {J. R.} and {Van Kuyk}, R. and Marx, {P. A.} and Gardner, {M. B.} and Luciw, {Paul A}",
year = "1991",
language = "English (US)",
volume = "7",
pages = "889--898",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "11",

}

TY - JOUR

T1 - Functional and immunological characterization of SIV envelope glycoprotein produced in genetically engineered mammalian cells

AU - Planelles, V.

AU - Haigwood, N. L.

AU - Marthas, Marta

AU - Mann, K. A.

AU - Scandella, C.

AU - Lidster, W. D.

AU - Shuster, J. R.

AU - Van Kuyk, R.

AU - Marx, P. A.

AU - Gardner, M. B.

AU - Luciw, Paul A

PY - 1991

Y1 - 1991

N2 - Retroviral envelope glycoproteins interact with cell receptors and are targets for antiviral immune responses in infected hosts. Macaque simian immunodeficiency virus (SIV(mac)) is a T-lymphocytopathic lentivirus which causes an AIDS-like disease in rhesus macaques. The envelope gene of SIV(mac) encodes a precursor glycoprotein (gp160) which is cleaved into an external domain (gp130) and a transmembrane domain (gp32). To investigate the functional and immunological properties of the SIV external envelope glycoprotein, we have used genetically engineered mammalian cells to produce recombinant gp130 (rgp130). The rgp130 has the appropriate molecular weight, is glycosylated, and has native conformation as determined by binding to the cell receptor for SIV, the CD4 antigen. Rhesus macaques immunized with purified rgp130 formulated in muramyl dipeptide adjuvant generated high titers of antienvelope antibodies. Antibodies from thse macaques were tested for in vitro virus neutralization; very low or undetectable levels of neutralization were observed. In contrast, neutralizing antibodies were readily detected in sera from goats immunized with rgp130. With respect to cell-mediated immunity, proliferative responses to rgp130 were demonstrated in peripheral blood monocyte cells (PBMC) from macaques immunized with the recombinant glycoprotein as well as in PBMC from SIV-infected animals. These results show that rgp130 is functional and immunogenic; the potential of rgp130 for protective immunization remains to be determined.

AB - Retroviral envelope glycoproteins interact with cell receptors and are targets for antiviral immune responses in infected hosts. Macaque simian immunodeficiency virus (SIV(mac)) is a T-lymphocytopathic lentivirus which causes an AIDS-like disease in rhesus macaques. The envelope gene of SIV(mac) encodes a precursor glycoprotein (gp160) which is cleaved into an external domain (gp130) and a transmembrane domain (gp32). To investigate the functional and immunological properties of the SIV external envelope glycoprotein, we have used genetically engineered mammalian cells to produce recombinant gp130 (rgp130). The rgp130 has the appropriate molecular weight, is glycosylated, and has native conformation as determined by binding to the cell receptor for SIV, the CD4 antigen. Rhesus macaques immunized with purified rgp130 formulated in muramyl dipeptide adjuvant generated high titers of antienvelope antibodies. Antibodies from thse macaques were tested for in vitro virus neutralization; very low or undetectable levels of neutralization were observed. In contrast, neutralizing antibodies were readily detected in sera from goats immunized with rgp130. With respect to cell-mediated immunity, proliferative responses to rgp130 were demonstrated in peripheral blood monocyte cells (PBMC) from macaques immunized with the recombinant glycoprotein as well as in PBMC from SIV-infected animals. These results show that rgp130 is functional and immunogenic; the potential of rgp130 for protective immunization remains to be determined.

UR - http://www.scopus.com/inward/record.url?scp=0025887590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025887590&partnerID=8YFLogxK

M3 - Article

C2 - 1760229

AN - SCOPUS:0025887590

VL - 7

SP - 889

EP - 898

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 11

ER -