Abstract
The osmotolerant yeast Zygosaccharomyces rouxii accumulates the polyols glycerol and D-arabitol intracellularly in response to hyperosmotic stress, but the membrane transport proteins regulating polyol accumulation have not been studied. We have cloned and characterized a FPS1 homologue in Z. rouxii NRRL Y2547, and its sequence revealed a 2709 bp open reading frame encoding a peptide of 692 deduced amino acids with 56.9% identity to the Saccharomyces cerevisiae Fps1p. The role of this putative membrane channel protein in polyol accumulation and release during osmoregulation was investigated. The Z. rouxii FPS1 (ZrFPS1) complemented the S. cerevisiae fps1Δ growth defect and glycerol release upon hypo-osmotic shock. Deletion of ZrFPS1 did not affect growth on glycerol as sole carbon source, suggesting that other transport proteins are involved in the uptake of glycerol. However, mutants lacking ZrFPS1 exhibited a significant decrease in glycerol and D-arabitol efflux and poor growth during hypo-osmotic conditions, suggesting that ZrFPS1 might be involved in D-arabitol transport in addition to glycerol. This is the first demonstration of a yeast gene that affects D-arabitol transport. The full-length ZrFPS1 gene sequence including upstream promoter has been deposited in the public database under Accession No. AY488133.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 571-581 |
| Number of pages | 11 |
| Journal | Yeast |
| Volume | 22 |
| Issue number | 7 |
| DOIs | |
| State | Published - May 2005 |
| Externally published | Yes |
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Keywords
- D-arabitol
- Fps1p
- Glycerol
- Osmoregulation
- Zygosaccharomyces rouxii
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Bioengineering
- Microbiology
Cite this
Functional analysis of the Zygosaccharomyces rouxii Fps1p homologue. / Tang, Xue Ming; Kayingo, Gerald; Prior, Bernard A.
In: Yeast, Vol. 22, No. 7, 05.2005, p. 571-581.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional analysis of the Zygosaccharomyces rouxii Fps1p homologue
AU - Tang, Xue Ming
AU - Kayingo, Gerald
AU - Prior, Bernard A.
PY - 2005/5
Y1 - 2005/5
N2 - The osmotolerant yeast Zygosaccharomyces rouxii accumulates the polyols glycerol and D-arabitol intracellularly in response to hyperosmotic stress, but the membrane transport proteins regulating polyol accumulation have not been studied. We have cloned and characterized a FPS1 homologue in Z. rouxii NRRL Y2547, and its sequence revealed a 2709 bp open reading frame encoding a peptide of 692 deduced amino acids with 56.9% identity to the Saccharomyces cerevisiae Fps1p. The role of this putative membrane channel protein in polyol accumulation and release during osmoregulation was investigated. The Z. rouxii FPS1 (ZrFPS1) complemented the S. cerevisiae fps1Δ growth defect and glycerol release upon hypo-osmotic shock. Deletion of ZrFPS1 did not affect growth on glycerol as sole carbon source, suggesting that other transport proteins are involved in the uptake of glycerol. However, mutants lacking ZrFPS1 exhibited a significant decrease in glycerol and D-arabitol efflux and poor growth during hypo-osmotic conditions, suggesting that ZrFPS1 might be involved in D-arabitol transport in addition to glycerol. This is the first demonstration of a yeast gene that affects D-arabitol transport. The full-length ZrFPS1 gene sequence including upstream promoter has been deposited in the public database under Accession No. AY488133.
AB - The osmotolerant yeast Zygosaccharomyces rouxii accumulates the polyols glycerol and D-arabitol intracellularly in response to hyperosmotic stress, but the membrane transport proteins regulating polyol accumulation have not been studied. We have cloned and characterized a FPS1 homologue in Z. rouxii NRRL Y2547, and its sequence revealed a 2709 bp open reading frame encoding a peptide of 692 deduced amino acids with 56.9% identity to the Saccharomyces cerevisiae Fps1p. The role of this putative membrane channel protein in polyol accumulation and release during osmoregulation was investigated. The Z. rouxii FPS1 (ZrFPS1) complemented the S. cerevisiae fps1Δ growth defect and glycerol release upon hypo-osmotic shock. Deletion of ZrFPS1 did not affect growth on glycerol as sole carbon source, suggesting that other transport proteins are involved in the uptake of glycerol. However, mutants lacking ZrFPS1 exhibited a significant decrease in glycerol and D-arabitol efflux and poor growth during hypo-osmotic conditions, suggesting that ZrFPS1 might be involved in D-arabitol transport in addition to glycerol. This is the first demonstration of a yeast gene that affects D-arabitol transport. The full-length ZrFPS1 gene sequence including upstream promoter has been deposited in the public database under Accession No. AY488133.
KW - D-arabitol
KW - Fps1p
KW - Glycerol
KW - Osmoregulation
KW - Zygosaccharomyces rouxii
UR - http://www.scopus.com/inward/record.url?scp=21644435191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21644435191&partnerID=8YFLogxK
U2 - 10.1002/yea.1232
DO - 10.1002/yea.1232
M3 - Article
C2 - 15942934
AN - SCOPUS:21644435191
VL - 22
SP - 571
EP - 581
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 7
ER -