Abstract
Background & Aims The pathways regulating liver regeneration have been extensively studied within the liver. However, the signaling contribution derived from the gut microbiota to liver regeneration is poorly understood. Methods Microbiota and expression of hepatic genes in regenerating livers obtained from mice at 0 h to 9 days post 2/3 partial hepatectomy were temporally profiled to establish their interactive relationships. Results Partial hepatectomy led to rapid changes in gut microbiota that was reflected in an increased abundance of Bacteroidetes S24-7 and Rikenellaceae and decreased abundance of Firmicutes Clostridiales, Lachnospiraceae, and Ruminococcaceae. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to infer biological functional changes of the shifted microbiota. RNA-sequencing data revealed 6125 genes with more than a 2-fold difference in their expression levels during regeneration. By analyzing their expression pattern, six uniquely expressed patterns were observed. In addition, there were significant correlations between hepatic gene expression profiles and shifted bacterial populations during regeneration. Moreover, hepatic metabolism and immune function were closely associated with the abundance of Ruminococcacea, Lachnospiraceae, and S24-7. Bile acid profile was analyzed because bacterial enzymes produce bile acids that significantly impact hepatocyte proliferation. The data revealed that specific bacteria were closely associated with the concentration of certain bile acids and expression of hepatic genes. Conclusions The presented data established, for the first time, an intimate relationship between intestinal microbiota and the expression of hepatic genes in regenerating livers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 641-650 |
| Number of pages | 10 |
| Journal | Journal of Hepatology |
| Volume | 64 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2016 |
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Keywords
- Bile acid
- Gutliver axis
- Immune response
- Metabolism
- Partial hepatectomy
ASJC Scopus subject areas
- Medicine(all)
- Hepatology
Cite this
Functional analysis of the relationship between intestinal microbiota and the expression of hepatic genes and pathways during the course of liver regeneration. / Liu, Hui Xin; Rocha, Clarissa Santos; Dandekar, Satya; Wan, Yu-Jui Yvonne.
In: Journal of Hepatology, Vol. 64, No. 3, 01.03.2016, p. 641-650.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional analysis of the relationship between intestinal microbiota and the expression of hepatic genes and pathways during the course of liver regeneration
AU - Liu, Hui Xin
AU - Rocha, Clarissa Santos
AU - Dandekar, Satya
AU - Wan, Yu-Jui Yvonne
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Background & Aims The pathways regulating liver regeneration have been extensively studied within the liver. However, the signaling contribution derived from the gut microbiota to liver regeneration is poorly understood. Methods Microbiota and expression of hepatic genes in regenerating livers obtained from mice at 0 h to 9 days post 2/3 partial hepatectomy were temporally profiled to establish their interactive relationships. Results Partial hepatectomy led to rapid changes in gut microbiota that was reflected in an increased abundance of Bacteroidetes S24-7 and Rikenellaceae and decreased abundance of Firmicutes Clostridiales, Lachnospiraceae, and Ruminococcaceae. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to infer biological functional changes of the shifted microbiota. RNA-sequencing data revealed 6125 genes with more than a 2-fold difference in their expression levels during regeneration. By analyzing their expression pattern, six uniquely expressed patterns were observed. In addition, there were significant correlations between hepatic gene expression profiles and shifted bacterial populations during regeneration. Moreover, hepatic metabolism and immune function were closely associated with the abundance of Ruminococcacea, Lachnospiraceae, and S24-7. Bile acid profile was analyzed because bacterial enzymes produce bile acids that significantly impact hepatocyte proliferation. The data revealed that specific bacteria were closely associated with the concentration of certain bile acids and expression of hepatic genes. Conclusions The presented data established, for the first time, an intimate relationship between intestinal microbiota and the expression of hepatic genes in regenerating livers.
AB - Background & Aims The pathways regulating liver regeneration have been extensively studied within the liver. However, the signaling contribution derived from the gut microbiota to liver regeneration is poorly understood. Methods Microbiota and expression of hepatic genes in regenerating livers obtained from mice at 0 h to 9 days post 2/3 partial hepatectomy were temporally profiled to establish their interactive relationships. Results Partial hepatectomy led to rapid changes in gut microbiota that was reflected in an increased abundance of Bacteroidetes S24-7 and Rikenellaceae and decreased abundance of Firmicutes Clostridiales, Lachnospiraceae, and Ruminococcaceae. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to infer biological functional changes of the shifted microbiota. RNA-sequencing data revealed 6125 genes with more than a 2-fold difference in their expression levels during regeneration. By analyzing their expression pattern, six uniquely expressed patterns were observed. In addition, there were significant correlations between hepatic gene expression profiles and shifted bacterial populations during regeneration. Moreover, hepatic metabolism and immune function were closely associated with the abundance of Ruminococcacea, Lachnospiraceae, and S24-7. Bile acid profile was analyzed because bacterial enzymes produce bile acids that significantly impact hepatocyte proliferation. The data revealed that specific bacteria were closely associated with the concentration of certain bile acids and expression of hepatic genes. Conclusions The presented data established, for the first time, an intimate relationship between intestinal microbiota and the expression of hepatic genes in regenerating livers.
KW - Bile acid
KW - Gutliver axis
KW - Immune response
KW - Metabolism
KW - Partial hepatectomy
UR - http://www.scopus.com/inward/record.url?scp=84958841171&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84958841171&partnerID=8YFLogxK
U2 - 10.1016/j.jhep.2015.09.022
DO - 10.1016/j.jhep.2015.09.022
M3 - Article
VL - 64
SP - 641
EP - 650
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 3
ER -