Functional activity of the adenosine binding enhancer, PD 81,723, in the in vitro hippocampal slice

Cynthia A. Janusz, Robert F. Bruns, Robert F Berman

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The adenosine receptor binding enhancer, PD 81,723, enhances the inhibitory effects of exogenously applied adenosine in a dose-dependent manner in hippocampal brain slices. Extracellular recordings were obtained from the CA1 cell layer while electrically stimulating the stratum radiatum. Application of 1, 10 or 32 μM PD 81,723 in the presence of adenosine resulted in a dose-dependent reduction in the amplitude of the population spike which could be partially reversed by theophylline. In addition, hippocampal slices exposed to adenosine showed greater paired-pulse facilitation compared to control and this facilitation was significantly enhanced by the presence of PD 81,723. PD 81,723 had no effect when administered alone, but required the presence of adenosine. These results demonstrate that in addition to enhancing adenosine receptor binding, PD 81,723 also enhances the functional activity of adenosine in the hippocampal slice.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalBrain Research
Volume567
Issue number2
DOIs
StatePublished - Dec 20 1991
Externally publishedYes

Keywords

  • Adenosine receptor
  • Binding enhancer
  • Electrophysiology
  • Hippocampal slice
  • Receptor modulator
  • Theophylline

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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