Full range physiological mass transport control in 3D tissue cultures

Yu Hsiang Hsu, Monica L. Moya, Parinaz Abiri, Christopher C.W. Hughes, Steven George, Abraham P. Lee

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We report the first demonstration of a microfluidic platform that captures the full physiological range of mass transport in 3-D tissue culture. The basis of our method used long microfluidic channels connected to both sides of a central microtissue chamber at different downstream positions to control the mass transport distribution within the chamber. Precise control of the Péclet number (Pe), defined as the ratio of convective to diffusive transport, over nearly five orders of magnitude (0.0056 to 160) was achieved. The platform was used to systematically investigate the role of physiological mass transport on vasculogenesis. We demonstrate, for the first time, that vasculogenesis can be independently stimulated by interstitial flow (Pe > 10) or hypoxic conditions (Pe < 0.1), and not by the intermediate state (normal living tissue). This simple platform can be applied to physiological and biological studies of 3D living tissue followed by pathological disease studies, such as cancer research and drug screening.

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalLab on a Chip
Volume13
Issue number1
DOIs
StatePublished - Jan 7 2013

Fingerprint

Tissue culture
Microfluidics
Mass transfer
Tissue
Preclinical Drug Evaluations
Early Detection of Cancer
Screening
Demonstrations
Research
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Hsu, Y. H., Moya, M. L., Abiri, P., Hughes, C. C. W., George, S., & Lee, A. P. (2013). Full range physiological mass transport control in 3D tissue cultures. Lab on a Chip, 13(1), 81-89. https://doi.org/10.1039/c2lc40787f

Full range physiological mass transport control in 3D tissue cultures. / Hsu, Yu Hsiang; Moya, Monica L.; Abiri, Parinaz; Hughes, Christopher C.W.; George, Steven; Lee, Abraham P.

In: Lab on a Chip, Vol. 13, No. 1, 07.01.2013, p. 81-89.

Research output: Contribution to journalArticle

Hsu, YH, Moya, ML, Abiri, P, Hughes, CCW, George, S & Lee, AP 2013, 'Full range physiological mass transport control in 3D tissue cultures', Lab on a Chip, vol. 13, no. 1, pp. 81-89. https://doi.org/10.1039/c2lc40787f
Hsu, Yu Hsiang ; Moya, Monica L. ; Abiri, Parinaz ; Hughes, Christopher C.W. ; George, Steven ; Lee, Abraham P. / Full range physiological mass transport control in 3D tissue cultures. In: Lab on a Chip. 2013 ; Vol. 13, No. 1. pp. 81-89.
@article{f82182ecf1c4454ea696dfab0c613747,
title = "Full range physiological mass transport control in 3D tissue cultures",
abstract = "We report the first demonstration of a microfluidic platform that captures the full physiological range of mass transport in 3-D tissue culture. The basis of our method used long microfluidic channels connected to both sides of a central microtissue chamber at different downstream positions to control the mass transport distribution within the chamber. Precise control of the P{\'e}clet number (Pe), defined as the ratio of convective to diffusive transport, over nearly five orders of magnitude (0.0056 to 160) was achieved. The platform was used to systematically investigate the role of physiological mass transport on vasculogenesis. We demonstrate, for the first time, that vasculogenesis can be independently stimulated by interstitial flow (Pe > 10) or hypoxic conditions (Pe < 0.1), and not by the intermediate state (normal living tissue). This simple platform can be applied to physiological and biological studies of 3D living tissue followed by pathological disease studies, such as cancer research and drug screening.",
author = "Hsu, {Yu Hsiang} and Moya, {Monica L.} and Parinaz Abiri and Hughes, {Christopher C.W.} and Steven George and Lee, {Abraham P.}",
year = "2013",
month = "1",
day = "7",
doi = "10.1039/c2lc40787f",
language = "English (US)",
volume = "13",
pages = "81--89",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "1",

}

TY - JOUR

T1 - Full range physiological mass transport control in 3D tissue cultures

AU - Hsu, Yu Hsiang

AU - Moya, Monica L.

AU - Abiri, Parinaz

AU - Hughes, Christopher C.W.

AU - George, Steven

AU - Lee, Abraham P.

PY - 2013/1/7

Y1 - 2013/1/7

N2 - We report the first demonstration of a microfluidic platform that captures the full physiological range of mass transport in 3-D tissue culture. The basis of our method used long microfluidic channels connected to both sides of a central microtissue chamber at different downstream positions to control the mass transport distribution within the chamber. Precise control of the Péclet number (Pe), defined as the ratio of convective to diffusive transport, over nearly five orders of magnitude (0.0056 to 160) was achieved. The platform was used to systematically investigate the role of physiological mass transport on vasculogenesis. We demonstrate, for the first time, that vasculogenesis can be independently stimulated by interstitial flow (Pe > 10) or hypoxic conditions (Pe < 0.1), and not by the intermediate state (normal living tissue). This simple platform can be applied to physiological and biological studies of 3D living tissue followed by pathological disease studies, such as cancer research and drug screening.

AB - We report the first demonstration of a microfluidic platform that captures the full physiological range of mass transport in 3-D tissue culture. The basis of our method used long microfluidic channels connected to both sides of a central microtissue chamber at different downstream positions to control the mass transport distribution within the chamber. Precise control of the Péclet number (Pe), defined as the ratio of convective to diffusive transport, over nearly five orders of magnitude (0.0056 to 160) was achieved. The platform was used to systematically investigate the role of physiological mass transport on vasculogenesis. We demonstrate, for the first time, that vasculogenesis can be independently stimulated by interstitial flow (Pe > 10) or hypoxic conditions (Pe < 0.1), and not by the intermediate state (normal living tissue). This simple platform can be applied to physiological and biological studies of 3D living tissue followed by pathological disease studies, such as cancer research and drug screening.

UR - http://www.scopus.com/inward/record.url?scp=84870223467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870223467&partnerID=8YFLogxK

U2 - 10.1039/c2lc40787f

DO - 10.1039/c2lc40787f

M3 - Article

C2 - 23090158

AN - SCOPUS:84870223467

VL - 13

SP - 81

EP - 89

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 1

ER -