Full range physiological mass transport control in 3D tissue cultures

Yu Hsiang Hsu, Monica L. Moya, Parinaz Abiri, Christopher C.W. Hughes, Steven George, Abraham P. Lee

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

We report the first demonstration of a microfluidic platform that captures the full physiological range of mass transport in 3-D tissue culture. The basis of our method used long microfluidic channels connected to both sides of a central microtissue chamber at different downstream positions to control the mass transport distribution within the chamber. Precise control of the Péclet number (Pe), defined as the ratio of convective to diffusive transport, over nearly five orders of magnitude (0.0056 to 160) was achieved. The platform was used to systematically investigate the role of physiological mass transport on vasculogenesis. We demonstrate, for the first time, that vasculogenesis can be independently stimulated by interstitial flow (Pe > 10) or hypoxic conditions (Pe < 0.1), and not by the intermediate state (normal living tissue). This simple platform can be applied to physiological and biological studies of 3D living tissue followed by pathological disease studies, such as cancer research and drug screening.

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalLab on a Chip
Volume13
Issue number1
DOIs
StatePublished - Jan 7 2013

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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  • Cite this

    Hsu, Y. H., Moya, M. L., Abiri, P., Hughes, C. C. W., George, S., & Lee, A. P. (2013). Full range physiological mass transport control in 3D tissue cultures. Lab on a Chip, 13(1), 81-89. https://doi.org/10.1039/c2lc40787f