From combinatorial chemistry to chemical microarray

Kit Lam, Manat Renil

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

Combinatorial chemistry was first applied to the generation of peptide arrays in 1984. Since then, the field of combinatorial chemistry has evolved rapidly into a new discipline. There is a great need for the development of methods to examine the proteome functionally at a global level. Using many of the techniques and instruments developed for DNA microarrays, chemical microarray methods have advanced significantly in the past three years. High-density chemical microarrays can now be synthesized in situ on glass slides or be printed through covalent linkage or non-specific adsorption to the surface of the solid-support with fully automatic arrayers. Microfabrication methods enable one to generate arrays of microsensors at the end of optical fibers or arrays of microwells on a flat surface. In conjunction with the one-bead one-compound combinatorial library method, chemical microarrays have proven to be very useful in lead identification and optimization. High-throughput protein expression systems, robust high-density protein, peptide and small-molecule microarray systems, and automatic mass spectrometers are critical tools for the field of functional proteomics.

Original languageEnglish (US)
Pages (from-to)353-358
Number of pages6
JournalCurrent Opinion in Chemical Biology
Volume6
Issue number3
DOIs
StatePublished - Jun 1 2002

ASJC Scopus subject areas

  • Biochemistry

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