From combinatorial chemistry to chemical microarray

Kit Lam, Manat Renil

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Combinatorial chemistry was first applied to the generation of peptide arrays in 1984. Since then, the field of combinatorial chemistry has evolved rapidly into a new discipline. There is a great need for the development of methods to examine the proteome functionally at a global level. Using many of the techniques and instruments developed for DNA microarrays, chemical microarray methods have advanced significantly in the past three years. High-density chemical microarrays can now be synthesized in situ on glass slides or be printed through covalent linkage or non-specific adsorption to the surface of the solid-support with fully automatic arrayers. Microfabrication methods enable one to generate arrays of microsensors at the end of optical fibers or arrays of microwells on a flat surface. In conjunction with the one-bead one-compound combinatorial library method, chemical microarrays have proven to be very useful in lead identification and optimization. High-throughput protein expression systems, robust high-density protein, peptide and small-molecule microarray systems, and automatic mass spectrometers are critical tools for the field of functional proteomics.

Original languageEnglish (US)
Pages (from-to)353-358
Number of pages6
JournalCurrent Opinion in Chemical Biology
Volume6
Issue number3
DOIs
StatePublished - Jun 1 2002

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'From combinatorial chemistry to chemical microarray'. Together they form a unique fingerprint.

  • Cite this