From combinatorial chemistry to cancer-targeting peptides

Olulanu H. Aina, Ruiwu Liu, Julie Sutcliffe, Jan Marik, Chong-Xian Pan, Kit Lam

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

Several monoclonal antibodies that target cell surface receptors have gained approval by the U.S. Food and Drug Administration and are widely used in the treatment of some cancers. These include but are not limited to the anti-CD20 antibody Rituximab, used in lymphoma treatment, as well as anti-HER-2 antibody for breast cancer therapy. The efficacy of this cancer immunotherapy modality is, however, limited by the large size of the antibody (160 kd) and its relatively nonspecific binding to the reticuloendothelial system. This latter property is particularly problematic if the antibody is used as a vehicle to deliver radionuclides, cytotoxic drugs, or toxins to the tumor site. Peptides, peptidomimetic, or small molecules are thus attractive as alternative cell surface targeting agents for cancer imaging and therapy. Cancer cell surface targeting peptides can be derived from known native peptide hormones such as somatostatin and bombesin, or they can be identified through screening combinatorial peptide libraries against unknown cell surface receptor targets. Phage-display peptide library and one-bead one-compound (OBOC) combinatorial library methods have been successfully used to discover peptides that target cancer cells or tumor blood vessel endothelial cells. The phage-display peptide library method, because of its biological nature, can only display L-amino acid peptides. In contrast, the OBOC combinatorial library method allows for bead-surface display of peptides that contain L-amino acids, D-amino acids, unnatural amino acids, or other organic moieties. We have successfully used the OBOC method to discover and optimize ligands against unique cell surface receptors of prostate cancer, T- and B-cell lymphoma, as well as ovarian and lung cancers, and we have used some of these peptides to image xenografts in nude mice with high specificity. Here, we (i) review the literature on the use of phage-display and OBOC combinatorial library methods to discover cancer and tumor blood vessel targeting ligands, and (ii) report on the use of an ovarian cancer targeting ligand, OA02, as an in vivo PET imaging probe in a xenograft model in nude mice.

Original languageEnglish (US)
Pages (from-to)631-651
Number of pages21
JournalMolecular Pharmaceutics
Volume4
Issue number5
DOIs
StatePublished - Sep 2007

Keywords

  • Cancer cell surface targeting agents
  • Combinatorial chemistry
  • Integrin
  • On-bead cell binding
  • One-bead one-compound library
  • Optical imaging
  • Peptide library
  • PET imaging
  • Phage-display

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science

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