FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility

Nicoletta C. Surdo, Marco Berrera, Andreas Koschinski, Marcella Brescia, Matias R. Machado, Carolyn Carr, Peter Wright, Julia Gorelik, Stefano Morotti, Eleonora Grandi, Donald M Bers, Sergio Pantano, Manuela Zaccolo

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Compartmentalized cAMP/PKA signalling is now recognized as important for physiology and pathophysiology, yet a detailed understanding of the properties, regulation and function of local cAMP/PKA signals is lacking. Here we present a fluorescence resonance energy transfer (FRET)-based sensor, CUTie, which detects compartmentalized cAMP with unprecedented accuracy. CUTie, targeted to specific multiprotein complexes at discrete plasmalemmal, sarcoplasmic reticular and myofilament sites, reveals differential kinetics and amplitudes of localized cAMP signals. This nanoscopic heterogeneity of cAMP signals is necessary to optimize cardiac contractility upon adrenergic activation. At low adrenergic levels, and those mimicking heart failure, differential local cAMP responses are exacerbated, with near abolition of cAMP signalling at certain locations. This work provides tools and fundamental mechanistic insights into subcellular adrenergic signalling in normal and pathological cardiac function.

Original languageEnglish (US)
Pages (from-to)15031
Number of pages1
JournalNature Communications
DOIs
StateAccepted/In press - 2017

Fingerprint

adrenergics
Fluorescence Resonance Energy Transfer
Biosensing Techniques
resonance fluorescence
bioinstrumentation
Biosensors
Adrenergic Agents
Tuning
energy transfer
tuning
Multiprotein Complexes
physiology
Myofibrils
Physiology
Heart Failure
Chemical activation
activation
Kinetics
sensors
Sensors

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Surdo, N. C., Berrera, M., Koschinski, A., Brescia, M., Machado, M. R., Carr, C., ... Zaccolo, M. (Accepted/In press). FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility. Nature Communications, 15031. https://doi.org/10.1038/ncomms15031

FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility. / Surdo, Nicoletta C.; Berrera, Marco; Koschinski, Andreas; Brescia, Marcella; Machado, Matias R.; Carr, Carolyn; Wright, Peter; Gorelik, Julia; Morotti, Stefano; Grandi, Eleonora; Bers, Donald M; Pantano, Sergio; Zaccolo, Manuela.

In: Nature Communications, 2017, p. 15031.

Research output: Contribution to journalArticle

Surdo, NC, Berrera, M, Koschinski, A, Brescia, M, Machado, MR, Carr, C, Wright, P, Gorelik, J, Morotti, S, Grandi, E, Bers, DM, Pantano, S & Zaccolo, M 2017, 'FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility', Nature Communications, pp. 15031. https://doi.org/10.1038/ncomms15031
Surdo, Nicoletta C. ; Berrera, Marco ; Koschinski, Andreas ; Brescia, Marcella ; Machado, Matias R. ; Carr, Carolyn ; Wright, Peter ; Gorelik, Julia ; Morotti, Stefano ; Grandi, Eleonora ; Bers, Donald M ; Pantano, Sergio ; Zaccolo, Manuela. / FRET biosensor uncovers cAMP nano-domains at β-adrenergic targets that dictate precise tuning of cardiac contractility. In: Nature Communications. 2017 ; pp. 15031.
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