FRET-based trilateration of probes bound within functional ryanodine receptors

Bengt Svensson, Tetsuro Oda, Florentin R. Nitu, Yi Yang, Iustin Cornea, Ye Chen-Izu, James D. Fessenden, Donald M Bers, David D. Thomas, Razvan L. Cornea

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

To locate the biosensor peptide DPc10 bound to ryanodine receptor (RyR) Ca2+ channels, we developed an approach that combines fluorescence resonance energy transfer (FRET), simulated-annealing, cryo-electron microscopy, and crystallographic data. DPc10 is identical to the 2460-2495 segment within the cardiac muscle RyR isoform (RyR2) central domain. DPc10 binding to RyR2 results in a pathologically elevated Ca2+ leak by destabilizing key interactions between the RyR2 N-terminal and central domains (unzipping). To localize the DPc10 binding site within RyR2, we measured FRET between five single-cysteine variants of the FK506-binding protein (FKBP) labeled with a donor probe, and DPc10 labeled with an acceptor probe (A-DPc10). Effective donor positions were calculated from simulated-annealing constrained by both the RyR cryo-EM map and the FKBP atomic structure docked to the RyR. FRET to A-DPc10 was measured in permeabilized cardiomyocytes via confocal microscopy, converted to distances, and used to trilaterate the acceptor locus within RyR. Additional FRET measurements between donor-labeled calmodulin and A-DPc10 were used to constrain the trilaterations. Results locate the DPc10 probe within RyR domain 3, ∼35 Å from the previously docked N-terminal domain crystal structure. This multiscale approach may be useful in mapping other RyR sites of mechanistic interest within FRET range of FKBP.

Original languageEnglish (US)
Pages (from-to)2037-2048
Number of pages12
JournalBiophysical Journal
Volume107
Issue number9
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'FRET-based trilateration of probes bound within functional ryanodine receptors'. Together they form a unique fingerprint.

Cite this