Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model

Nisha K. Duggal, Jana M. Ritter, Samuel E. Pestorius, Sherif R. Zaki, Brent S. Davis, Gwong Jen J. Chang, Richard A. Bowen, Aaron Brault

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Circulation of Zika virus (ZIKV) was first identified in the Western hemisphere in late 2014. Primarily transmitted through mosquito bite, ZIKV can also be transmitted through sex and from mother to fetus, and maternal ZIKV infection has been associated with fetal malformations. We assessed immunodeficient AG129 mice for their capacity to shed ZIKV in semen and to infect female mice via sexual transmission. Infectious virus was detected in semen between 7 and 21 days post-inoculation, and ZIKV RNA was detected in semen through 58 days post-inoculation. During mating, 73% of infected males transmitted ZIKV to uninfected females, and 50% of females became infected, with evidence of fetal infection in resulting pregnancies. Semen from vasectomized mice contained significantly lower levels of infectious virus, though sexual transmission still occurred. This model provides a platform for studying the kinetics of ZIKV sexual transmission and prolonged RNA shedding also observed in human semen.

Original languageEnglish (US)
Pages (from-to)1751-1760
Number of pages10
JournalCell Reports
Volume18
Issue number7
DOIs
StatePublished - Feb 14 2017
Externally publishedYes

Fingerprint

Virus Shedding
Viral RNA
Viruses
Semen
RNA
Bites and Stings
Culicidae
Zika Virus
Fetus
Mothers
Pregnancy
Infection
Kinetics

Keywords

  • animal model
  • mouse model
  • pregnancy
  • semen
  • sexual transmission
  • vasectomy
  • Zika virus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Duggal, N. K., Ritter, J. M., Pestorius, S. E., Zaki, S. R., Davis, B. S., Chang, G. J. J., ... Brault, A. (2017). Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model. Cell Reports, 18(7), 1751-1760. https://doi.org/10.1016/j.celrep.2017.01.056

Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model. / Duggal, Nisha K.; Ritter, Jana M.; Pestorius, Samuel E.; Zaki, Sherif R.; Davis, Brent S.; Chang, Gwong Jen J.; Bowen, Richard A.; Brault, Aaron.

In: Cell Reports, Vol. 18, No. 7, 14.02.2017, p. 1751-1760.

Research output: Contribution to journalArticle

Duggal, NK, Ritter, JM, Pestorius, SE, Zaki, SR, Davis, BS, Chang, GJJ, Bowen, RA & Brault, A 2017, 'Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model', Cell Reports, vol. 18, no. 7, pp. 1751-1760. https://doi.org/10.1016/j.celrep.2017.01.056
Duggal, Nisha K. ; Ritter, Jana M. ; Pestorius, Samuel E. ; Zaki, Sherif R. ; Davis, Brent S. ; Chang, Gwong Jen J. ; Bowen, Richard A. ; Brault, Aaron. / Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model. In: Cell Reports. 2017 ; Vol. 18, No. 7. pp. 1751-1760.
@article{0974c30df0214a9db92a46b37d21f905,
title = "Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model",
abstract = "Circulation of Zika virus (ZIKV) was first identified in the Western hemisphere in late 2014. Primarily transmitted through mosquito bite, ZIKV can also be transmitted through sex and from mother to fetus, and maternal ZIKV infection has been associated with fetal malformations. We assessed immunodeficient AG129 mice for their capacity to shed ZIKV in semen and to infect female mice via sexual transmission. Infectious virus was detected in semen between 7 and 21 days post-inoculation, and ZIKV RNA was detected in semen through 58 days post-inoculation. During mating, 73{\%} of infected males transmitted ZIKV to uninfected females, and 50{\%} of females became infected, with evidence of fetal infection in resulting pregnancies. Semen from vasectomized mice contained significantly lower levels of infectious virus, though sexual transmission still occurred. This model provides a platform for studying the kinetics of ZIKV sexual transmission and prolonged RNA shedding also observed in human semen.",
keywords = "animal model, mouse model, pregnancy, semen, sexual transmission, vasectomy, Zika virus",
author = "Duggal, {Nisha K.} and Ritter, {Jana M.} and Pestorius, {Samuel E.} and Zaki, {Sherif R.} and Davis, {Brent S.} and Chang, {Gwong Jen J.} and Bowen, {Richard A.} and Aaron Brault",
year = "2017",
month = "2",
day = "14",
doi = "10.1016/j.celrep.2017.01.056",
language = "English (US)",
volume = "18",
pages = "1751--1760",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "7",

}

TY - JOUR

T1 - Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model

AU - Duggal, Nisha K.

AU - Ritter, Jana M.

AU - Pestorius, Samuel E.

AU - Zaki, Sherif R.

AU - Davis, Brent S.

AU - Chang, Gwong Jen J.

AU - Bowen, Richard A.

AU - Brault, Aaron

PY - 2017/2/14

Y1 - 2017/2/14

N2 - Circulation of Zika virus (ZIKV) was first identified in the Western hemisphere in late 2014. Primarily transmitted through mosquito bite, ZIKV can also be transmitted through sex and from mother to fetus, and maternal ZIKV infection has been associated with fetal malformations. We assessed immunodeficient AG129 mice for their capacity to shed ZIKV in semen and to infect female mice via sexual transmission. Infectious virus was detected in semen between 7 and 21 days post-inoculation, and ZIKV RNA was detected in semen through 58 days post-inoculation. During mating, 73% of infected males transmitted ZIKV to uninfected females, and 50% of females became infected, with evidence of fetal infection in resulting pregnancies. Semen from vasectomized mice contained significantly lower levels of infectious virus, though sexual transmission still occurred. This model provides a platform for studying the kinetics of ZIKV sexual transmission and prolonged RNA shedding also observed in human semen.

AB - Circulation of Zika virus (ZIKV) was first identified in the Western hemisphere in late 2014. Primarily transmitted through mosquito bite, ZIKV can also be transmitted through sex and from mother to fetus, and maternal ZIKV infection has been associated with fetal malformations. We assessed immunodeficient AG129 mice for their capacity to shed ZIKV in semen and to infect female mice via sexual transmission. Infectious virus was detected in semen between 7 and 21 days post-inoculation, and ZIKV RNA was detected in semen through 58 days post-inoculation. During mating, 73% of infected males transmitted ZIKV to uninfected females, and 50% of females became infected, with evidence of fetal infection in resulting pregnancies. Semen from vasectomized mice contained significantly lower levels of infectious virus, though sexual transmission still occurred. This model provides a platform for studying the kinetics of ZIKV sexual transmission and prolonged RNA shedding also observed in human semen.

KW - animal model

KW - mouse model

KW - pregnancy

KW - semen

KW - sexual transmission

KW - vasectomy

KW - Zika virus

UR - http://www.scopus.com/inward/record.url?scp=85013158360&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013158360&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2017.01.056

DO - 10.1016/j.celrep.2017.01.056

M3 - Article

VL - 18

SP - 1751

EP - 1760

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 7

ER -