Frequency dependent effects of oscillating fluid flow on bone cells

Jun You, Marnie Saunders, Clare E Yellowley-genetos, Henry Donahue, Christopher Jacobs

Research output: Contribution to journalArticlepeer-review


A high-frequency flow device was developed to deliver a wide range of sinusoidally oscillating flow frequencies while monitoring real time [ Ca2+]i and PGE2 production in MC3T3-E1 osteoblastic cells. Results show that the more stimulatory frequency for [Ca2+]i was 0.1 Hz. However, 0.5-1.0 Hz was more stimulatory of PGE2 production. This suggests that the signaling pathways for [Ca2+]i and PGE2 may be different. Moreover considering viscoelastic properties of cells, results suggest that bone cell [ Ca2+]i response is more dependent on cell membrane deformation whereas PGE2 production is more related to cytosol internal friction.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Issue numberSUPPL. 1
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering


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