A high-frequency flow device was developed to deliver a wide range of sinusoidally oscillating flow frequencies while monitoring real time [ Ca2+]i and PGE2 production in MC3T3-E1 osteoblastic cells. Results show that the more stimulatory frequency for [Ca2+]i was 0.1 Hz. However, 0.5-1.0 Hz was more stimulatory of PGE2 production. This suggests that the signaling pathways for [Ca2+]i and PGE2 may be different. Moreover considering viscoelastic properties of cells, results suggest that bone cell [ Ca2+]i response is more dependent on cell membrane deformation whereas PGE2 production is more related to cytosol internal friction.
|Original language||English (US)|
|Journal||Annals of Biomedical Engineering|
|Issue number||SUPPL. 1|
|Publication status||Published - 2000|
ASJC Scopus subject areas
- Biomedical Engineering