Abstract
A high-frequency flow device was developed to deliver a wide range of sinusoidally oscillating flow frequencies while monitoring real time [ Ca2+]i and PGE2 production in MC3T3-E1 osteoblastic cells. Results show that the more stimulatory frequency for [Ca2+]i was 0.1 Hz. However, 0.5-1.0 Hz was more stimulatory of PGE2 production. This suggests that the signaling pathways for [Ca2+]i and PGE2 may be different. Moreover considering viscoelastic properties of cells, results suggest that bone cell [ Ca2+]i response is more dependent on cell membrane deformation whereas PGE2 production is more related to cytosol internal friction.
| Original language | English (US) |
|---|---|
| Journal | Annals of Biomedical Engineering |
| Volume | 28 |
| Issue number | SUPPL. 1 |
| State | Published - 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Frequency dependent effects of oscillating fluid flow on bone cells. / You, Jun; Saunders, Marnie; Yellowley-genetos, Clare E; Donahue, Henry; Jacobs, Christopher.
In: Annals of Biomedical Engineering, Vol. 28, No. SUPPL. 1, 2000.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Frequency dependent effects of oscillating fluid flow on bone cells
AU - You, Jun
AU - Saunders, Marnie
AU - Yellowley-genetos, Clare E
AU - Donahue, Henry
AU - Jacobs, Christopher
PY - 2000
Y1 - 2000
N2 - A high-frequency flow device was developed to deliver a wide range of sinusoidally oscillating flow frequencies while monitoring real time [ Ca2+]i and PGE2 production in MC3T3-E1 osteoblastic cells. Results show that the more stimulatory frequency for [Ca2+]i was 0.1 Hz. However, 0.5-1.0 Hz was more stimulatory of PGE2 production. This suggests that the signaling pathways for [Ca2+]i and PGE2 may be different. Moreover considering viscoelastic properties of cells, results suggest that bone cell [ Ca2+]i response is more dependent on cell membrane deformation whereas PGE2 production is more related to cytosol internal friction.
AB - A high-frequency flow device was developed to deliver a wide range of sinusoidally oscillating flow frequencies while monitoring real time [ Ca2+]i and PGE2 production in MC3T3-E1 osteoblastic cells. Results show that the more stimulatory frequency for [Ca2+]i was 0.1 Hz. However, 0.5-1.0 Hz was more stimulatory of PGE2 production. This suggests that the signaling pathways for [Ca2+]i and PGE2 may be different. Moreover considering viscoelastic properties of cells, results suggest that bone cell [ Ca2+]i response is more dependent on cell membrane deformation whereas PGE2 production is more related to cytosol internal friction.
UR - http://www.scopus.com/inward/record.url?scp=0034504884&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034504884&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0034504884
VL - 28
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
SN - 0090-6964
IS - SUPPL. 1
ER -