Abstract
Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilization and rehydration. It was revealed that glucose is the most effective sugar amongst those tested, and the concentration of glucose was very sensitive and critical to the survival of red blood cells during lyophilization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 270-275 |
| Number of pages | 6 |
| Journal | Cell Preservation Technology |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
Cite this
Freeze-drying of human red blood cells : Influence of carbohydrates and their concentrations. / Yu, Jianping; Liu, J. H.; Pu, Lee Li-Qun; Cui, Xiangdong; Wang, Changzheng; Ouyang, S. L.; Gao, Dayong.
In: Cell Preservation Technology, Vol. 2, No. 4, 2004, p. 270-275.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Freeze-drying of human red blood cells
T2 - Influence of carbohydrates and their concentrations
AU - Yu, Jianping
AU - Liu, J. H.
AU - Pu, Lee Li-Qun
AU - Cui, Xiangdong
AU - Wang, Changzheng
AU - Ouyang, S. L.
AU - Gao, Dayong
PY - 2004
Y1 - 2004
N2 - Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilization and rehydration. It was revealed that glucose is the most effective sugar amongst those tested, and the concentration of glucose was very sensitive and critical to the survival of red blood cells during lyophilization.
AB - Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilization and rehydration. It was revealed that glucose is the most effective sugar amongst those tested, and the concentration of glucose was very sensitive and critical to the survival of red blood cells during lyophilization.
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UR - http://www.scopus.com/inward/citedby.url?scp=12344321678&partnerID=8YFLogxK
U2 - 10.1089/cpt.2004.2.270
DO - 10.1089/cpt.2004.2.270
M3 - Article
VL - 2
SP - 270
EP - 275
JO - Biopreservation and Biobanking
JF - Biopreservation and Biobanking
SN - 1947-5535
IS - 4
ER -