Freeze-drying of human red blood cells

Influence of carbohydrates and their concentrations

Jianping Yu, J. H. Liu, Lee Li-Qun Pu, Xiangdong Cui, Changzheng Wang, S. L. Ouyang, Dayong Gao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilization and rehydration. It was revealed that glucose is the most effective sugar amongst those tested, and the concentration of glucose was very sensitive and critical to the survival of red blood cells during lyophilization.

Original languageEnglish (US)
Pages (from-to)270-275
Number of pages6
JournalCell Preservation Technology
Volume2
Issue number4
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Freeze Drying
Drying
Blood
Erythrocytes
Cells
Carbohydrates
Sugars
Recovery
Fluid Therapy
Glucose
Platelets
Freight transportation
Biological Preservation
Biological materials
Polymers
Hemoglobins
Patents
Cryopreservation
Hematocrit
Cell Survival

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

Cite this

Freeze-drying of human red blood cells : Influence of carbohydrates and their concentrations. / Yu, Jianping; Liu, J. H.; Pu, Lee Li-Qun; Cui, Xiangdong; Wang, Changzheng; Ouyang, S. L.; Gao, Dayong.

In: Cell Preservation Technology, Vol. 2, No. 4, 2004, p. 270-275.

Research output: Contribution to journalArticle

Yu, Jianping ; Liu, J. H. ; Pu, Lee Li-Qun ; Cui, Xiangdong ; Wang, Changzheng ; Ouyang, S. L. ; Gao, Dayong. / Freeze-drying of human red blood cells : Influence of carbohydrates and their concentrations. In: Cell Preservation Technology. 2004 ; Vol. 2, No. 4. pp. 270-275.
@article{1e5feed00b214b2a9b20c1d442eafd33,
title = "Freeze-drying of human red blood cells: Influence of carbohydrates and their concentrations",
abstract = "Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilization and rehydration. It was revealed that glucose is the most effective sugar amongst those tested, and the concentration of glucose was very sensitive and critical to the survival of red blood cells during lyophilization.",
author = "Jianping Yu and Liu, {J. H.} and Pu, {Lee Li-Qun} and Xiangdong Cui and Changzheng Wang and Ouyang, {S. L.} and Dayong Gao",
year = "2004",
doi = "10.1089/cpt.2004.2.270",
language = "English (US)",
volume = "2",
pages = "270--275",
journal = "Biopreservation and Biobanking",
issn = "1947-5535",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Freeze-drying of human red blood cells

T2 - Influence of carbohydrates and their concentrations

AU - Yu, Jianping

AU - Liu, J. H.

AU - Pu, Lee Li-Qun

AU - Cui, Xiangdong

AU - Wang, Changzheng

AU - Ouyang, S. L.

AU - Gao, Dayong

PY - 2004

Y1 - 2004

N2 - Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilization and rehydration. It was revealed that glucose is the most effective sugar amongst those tested, and the concentration of glucose was very sensitive and critical to the survival of red blood cells during lyophilization.

AB - Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilization and rehydration. It was revealed that glucose is the most effective sugar amongst those tested, and the concentration of glucose was very sensitive and critical to the survival of red blood cells during lyophilization.

UR - http://www.scopus.com/inward/record.url?scp=12344321678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12344321678&partnerID=8YFLogxK

U2 - 10.1089/cpt.2004.2.270

DO - 10.1089/cpt.2004.2.270

M3 - Article

VL - 2

SP - 270

EP - 275

JO - Biopreservation and Biobanking

JF - Biopreservation and Biobanking

SN - 1947-5535

IS - 4

ER -