Abstract
Historically, piscine francisellosis in various warm-, temperate-, and coldwater fish hosts has been attributed to Francisella noatunensis. From 2015 to 2016, an undescribed Francisella sp. was recovered during mortality events in cultured spotted rose snapper (Lutjanus guttatus) off the Pacific coast of Central America. Despite high mortality and emaciation, limited gross findings were observed in affected fish. Histological examination revealed multifocal granulomatous lesions, with the presence of numerous small, pleomorphic coccobacilli, predominantly in the peritoneum, spleen, kidneys, liver, pancreas, heart, and intestine. Sequencing of an ~1,400-bp fragment of the 16S rRNA gene demonstrated these isolates to be most similar (99.9% identity) to Francisella sp. isolate TX077308 cultured from seawater in the Gulf of Mexico, while sharing < 99% similarity to other Fransicella spp. Biochemical analysis, multilocus sequence comparisons of select housekeeping genes, repetitive extragenic palindromic PCR fingerprinting, matrix-assisted laser desorption ionization- time of flight mass spectrometry, and fatty acid methyl ester analysis revealed marked differences between these isolates and other described members of the genus. Koch's postulates were fulfilled by experimental intracoelomic injection and immersion trials using Nile (Oreochromis niloticus) and blue (Oreochromis aureus) tilapia. Based on observed phenotypic and genotypic differences from recognized Francisella spp., the name Francisella marina sp. nov. (NRRL B-65518) is proposed to accommodate these novel strains.
| Original language | English (US) |
|---|---|
| Article number | e00144-18 |
| Journal | Applied and Environmental Microbiology |
| Volume | 84 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 1 2018 |
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Keywords
- Aquaculture
- Fish pathogens
- Francisella
- Snapper
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
- Ecology
Cite this
Francisella marina sp. nov., etiologic agent of systemic disease in cultured spotted rose snapper (Lutjanus guttatus) in Central America. / Soto Martinez, Esteban; Griffin, Matt J.; Morales, Juan Alberto; Calvo, Elías Barquero; Sebastião, Fernanda de Alexandre; Porras, Adrián Lopez; Víquez-Rodríguez, Xindy; Reichley, Stephen R.; Rosser, Thomas G.; Ware, Cynthia; Byrne, Barbara A; García, Julio C.; LaFrentz, Benjamin R.; Camus, Alvin C.
In: Applied and Environmental Microbiology, Vol. 84, No. 16, e00144-18, 01.08.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Francisella marina sp. nov., etiologic agent of systemic disease in cultured spotted rose snapper (Lutjanus guttatus) in Central America
AU - Soto Martinez, Esteban
AU - Griffin, Matt J.
AU - Morales, Juan Alberto
AU - Calvo, Elías Barquero
AU - Sebastião, Fernanda de Alexandre
AU - Porras, Adrián Lopez
AU - Víquez-Rodríguez, Xindy
AU - Reichley, Stephen R.
AU - Rosser, Thomas G.
AU - Ware, Cynthia
AU - Byrne, Barbara A
AU - García, Julio C.
AU - LaFrentz, Benjamin R.
AU - Camus, Alvin C.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Historically, piscine francisellosis in various warm-, temperate-, and coldwater fish hosts has been attributed to Francisella noatunensis. From 2015 to 2016, an undescribed Francisella sp. was recovered during mortality events in cultured spotted rose snapper (Lutjanus guttatus) off the Pacific coast of Central America. Despite high mortality and emaciation, limited gross findings were observed in affected fish. Histological examination revealed multifocal granulomatous lesions, with the presence of numerous small, pleomorphic coccobacilli, predominantly in the peritoneum, spleen, kidneys, liver, pancreas, heart, and intestine. Sequencing of an ~1,400-bp fragment of the 16S rRNA gene demonstrated these isolates to be most similar (99.9% identity) to Francisella sp. isolate TX077308 cultured from seawater in the Gulf of Mexico, while sharing < 99% similarity to other Fransicella spp. Biochemical analysis, multilocus sequence comparisons of select housekeeping genes, repetitive extragenic palindromic PCR fingerprinting, matrix-assisted laser desorption ionization- time of flight mass spectrometry, and fatty acid methyl ester analysis revealed marked differences between these isolates and other described members of the genus. Koch's postulates were fulfilled by experimental intracoelomic injection and immersion trials using Nile (Oreochromis niloticus) and blue (Oreochromis aureus) tilapia. Based on observed phenotypic and genotypic differences from recognized Francisella spp., the name Francisella marina sp. nov. (NRRL B-65518) is proposed to accommodate these novel strains.
AB - Historically, piscine francisellosis in various warm-, temperate-, and coldwater fish hosts has been attributed to Francisella noatunensis. From 2015 to 2016, an undescribed Francisella sp. was recovered during mortality events in cultured spotted rose snapper (Lutjanus guttatus) off the Pacific coast of Central America. Despite high mortality and emaciation, limited gross findings were observed in affected fish. Histological examination revealed multifocal granulomatous lesions, with the presence of numerous small, pleomorphic coccobacilli, predominantly in the peritoneum, spleen, kidneys, liver, pancreas, heart, and intestine. Sequencing of an ~1,400-bp fragment of the 16S rRNA gene demonstrated these isolates to be most similar (99.9% identity) to Francisella sp. isolate TX077308 cultured from seawater in the Gulf of Mexico, while sharing < 99% similarity to other Fransicella spp. Biochemical analysis, multilocus sequence comparisons of select housekeeping genes, repetitive extragenic palindromic PCR fingerprinting, matrix-assisted laser desorption ionization- time of flight mass spectrometry, and fatty acid methyl ester analysis revealed marked differences between these isolates and other described members of the genus. Koch's postulates were fulfilled by experimental intracoelomic injection and immersion trials using Nile (Oreochromis niloticus) and blue (Oreochromis aureus) tilapia. Based on observed phenotypic and genotypic differences from recognized Francisella spp., the name Francisella marina sp. nov. (NRRL B-65518) is proposed to accommodate these novel strains.
KW - Aquaculture
KW - Fish pathogens
KW - Francisella
KW - Snapper
UR - http://www.scopus.com/inward/record.url?scp=85051700535&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051700535&partnerID=8YFLogxK
U2 - 10.1128/AEM.000144-18
DO - 10.1128/AEM.000144-18
M3 - Article
C2 - 29915103
AN - SCOPUS:85051700535
VL - 84
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 16
M1 - e00144-18
ER -