Francisella asiatica as the causative agent of piscine francisellosis in cultured tilapia (oreochromis sp.) in the united states

Esteban Soto Martinez, Wes Baumgartner, Judy Wiles, John P. Hawke

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Francisella asiatica is a Gram-negative, pleomorphic, facultative intracellular, bacterial pathogen that causes acute to chronic disease in a wide variety of warm-water cultured and wild fish species. Outbreaks of francisellosis in warm water fish have been documented in Taiwan, Japan, United Kingdom, Hawaii, and Latin America (including Costa Rica) but the organism has only been reported from the United States on one occasion from hybrid striped bass in California. In 2010, the bacterium was detected from diseased tilapia by culture on cystine heart agar supplemented with hemoglobin and by utilizing an F. asiatica-specific real-time polymerase chain reaction assay. The tilapia (Oreochromis niloticus) were cultured in an indoor, closed, recirculating aquaculture facility in the Midwest of the United States. The identity of isolates recovered from diseased fish was confirmed as F. asiatica by amplification and sequence comparison of the 16S ribosomal RNA and intracellular growth locus C (iglC) gene. Gross and microscopic examination of affected tissues revealed the presence of marked anterior renomegaly and splenomegaly with severe granulomatous disease.

Original languageEnglish (US)
Pages (from-to)821-825
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume23
Issue number4
DOIs
StatePublished - Jul 2011
Externally publishedYes

Fingerprint

Francisella
Tilapia
Oreochromis
tilapia (common name)
Fishes
Fish Diseases
16S Ribosomal RNA
Bass
Cichlids
Costa Rica
Fetal Hemoglobin
splenomegaly
Aquaculture
Cystine
Morone saxatilis
Midwestern United States
Latin America
Water
wild fish
Splenomegaly

Keywords

  • Francisella asiatica
  • granulomas
  • tilapia

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Francisella asiatica as the causative agent of piscine francisellosis in cultured tilapia (oreochromis sp.) in the united states. / Soto Martinez, Esteban; Baumgartner, Wes; Wiles, Judy; Hawke, John P.

In: Journal of Veterinary Diagnostic Investigation, Vol. 23, No. 4, 07.2011, p. 821-825.

Research output: Contribution to journalArticle

@article{dc891053fa7d413eb80f899fbf81ed41,
title = "Francisella asiatica as the causative agent of piscine francisellosis in cultured tilapia (oreochromis sp.) in the united states",
abstract = "Francisella asiatica is a Gram-negative, pleomorphic, facultative intracellular, bacterial pathogen that causes acute to chronic disease in a wide variety of warm-water cultured and wild fish species. Outbreaks of francisellosis in warm water fish have been documented in Taiwan, Japan, United Kingdom, Hawaii, and Latin America (including Costa Rica) but the organism has only been reported from the United States on one occasion from hybrid striped bass in California. In 2010, the bacterium was detected from diseased tilapia by culture on cystine heart agar supplemented with hemoglobin and by utilizing an F. asiatica-specific real-time polymerase chain reaction assay. The tilapia (Oreochromis niloticus) were cultured in an indoor, closed, recirculating aquaculture facility in the Midwest of the United States. The identity of isolates recovered from diseased fish was confirmed as F. asiatica by amplification and sequence comparison of the 16S ribosomal RNA and intracellular growth locus C (iglC) gene. Gross and microscopic examination of affected tissues revealed the presence of marked anterior renomegaly and splenomegaly with severe granulomatous disease.",
keywords = "Francisella asiatica, granulomas, tilapia",
author = "{Soto Martinez}, Esteban and Wes Baumgartner and Judy Wiles and Hawke, {John P.}",
year = "2011",
month = "7",
doi = "10.1177/1040638711407058",
language = "English (US)",
volume = "23",
pages = "821--825",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "4",

}

TY - JOUR

T1 - Francisella asiatica as the causative agent of piscine francisellosis in cultured tilapia (oreochromis sp.) in the united states

AU - Soto Martinez, Esteban

AU - Baumgartner, Wes

AU - Wiles, Judy

AU - Hawke, John P.

PY - 2011/7

Y1 - 2011/7

N2 - Francisella asiatica is a Gram-negative, pleomorphic, facultative intracellular, bacterial pathogen that causes acute to chronic disease in a wide variety of warm-water cultured and wild fish species. Outbreaks of francisellosis in warm water fish have been documented in Taiwan, Japan, United Kingdom, Hawaii, and Latin America (including Costa Rica) but the organism has only been reported from the United States on one occasion from hybrid striped bass in California. In 2010, the bacterium was detected from diseased tilapia by culture on cystine heart agar supplemented with hemoglobin and by utilizing an F. asiatica-specific real-time polymerase chain reaction assay. The tilapia (Oreochromis niloticus) were cultured in an indoor, closed, recirculating aquaculture facility in the Midwest of the United States. The identity of isolates recovered from diseased fish was confirmed as F. asiatica by amplification and sequence comparison of the 16S ribosomal RNA and intracellular growth locus C (iglC) gene. Gross and microscopic examination of affected tissues revealed the presence of marked anterior renomegaly and splenomegaly with severe granulomatous disease.

AB - Francisella asiatica is a Gram-negative, pleomorphic, facultative intracellular, bacterial pathogen that causes acute to chronic disease in a wide variety of warm-water cultured and wild fish species. Outbreaks of francisellosis in warm water fish have been documented in Taiwan, Japan, United Kingdom, Hawaii, and Latin America (including Costa Rica) but the organism has only been reported from the United States on one occasion from hybrid striped bass in California. In 2010, the bacterium was detected from diseased tilapia by culture on cystine heart agar supplemented with hemoglobin and by utilizing an F. asiatica-specific real-time polymerase chain reaction assay. The tilapia (Oreochromis niloticus) were cultured in an indoor, closed, recirculating aquaculture facility in the Midwest of the United States. The identity of isolates recovered from diseased fish was confirmed as F. asiatica by amplification and sequence comparison of the 16S ribosomal RNA and intracellular growth locus C (iglC) gene. Gross and microscopic examination of affected tissues revealed the presence of marked anterior renomegaly and splenomegaly with severe granulomatous disease.

KW - Francisella asiatica

KW - granulomas

KW - tilapia

UR - http://www.scopus.com/inward/record.url?scp=84855928591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855928591&partnerID=8YFLogxK

U2 - 10.1177/1040638711407058

DO - 10.1177/1040638711407058

M3 - Article

VL - 23

SP - 821

EP - 825

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 4

ER -