Foxj1 is required for apical localization of ezrin in airway epithelial cells

Tao Huang, You You, Melanie S. Spoor, Edward J. Richer, Vrinda V. Kudva, Renee C. Paige, Michael P. Seiler, Janice M. Liebler, Joseph Zabner, Charles Plopper, Steven L. Brody

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Establishment and maintenance of epithelial cell polarity depend on cytoskeletal organization and protein trafficking to polarized cortical membranes. ERM (ezrin, radixin, moesin) family members link polarized proteins with cytoskeletal actin. Although ERMs are often considered to be functionally similar, we found that, in airway epithelial cells, apical localization of ERMs depend on cell differentiation and is independently regulated. Moesin was present in the apical membrane of all undifferentiated epithelial cells. However, in differentiated cells, ezrin and moesin were selectively localized to apical membranes of ciliated airway cells and were absent from secretory cells. To identify regulatory proteins required for selective ERM trafficking, we evaluated airway epithelial cells lacking Foxj1, an F-box factor that directs programs required for cilia formation at the apical membrane. Interestingly, Foxj1 expression was also required for localization of apical ezrin, but not moesin. Additionally, membrane-cytoskeletal and threonine-phosphorylated ezrin were decreased in Foxj1-null cells, consistent with absent apical ezrin. Although apical moesin expression was present in null cells, it could not compensate for ezrin because ERM-associated EBP50 and the β2 adrenergic receptor failed to localize apically in the absence of Foxj1. These findings indicate that Foxj1 regulates ERM proteins differentially to selectively direct the apical localization of ezrin for the organization of multi-protein complexes in apical membranes of airway epithelial cells.

Original languageEnglish (US)
Pages (from-to)4935-4945
Number of pages11
JournalJournal of Cell Science
Volume116
Issue number24
DOIs
StatePublished - Dec 15 2003

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Epithelial Cells
Membranes
Null Lymphocytes
ezrin
Cell Polarity
Proteins
Cytoskeletal Proteins
moesin
Cilia
Protein Transport
Threonine
Adrenergic Receptors
Actins
Cell Differentiation
Maintenance
Organizations
radixin

Keywords

  • Cilia
  • Cytoskeleton
  • Differentiation
  • Lung
  • Mouse

ASJC Scopus subject areas

  • Cell Biology

Cite this

Huang, T., You, Y., Spoor, M. S., Richer, E. J., Kudva, V. V., Paige, R. C., ... Brody, S. L. (2003). Foxj1 is required for apical localization of ezrin in airway epithelial cells. Journal of Cell Science, 116(24), 4935-4945. https://doi.org/10.1242/jcs.00830

Foxj1 is required for apical localization of ezrin in airway epithelial cells. / Huang, Tao; You, You; Spoor, Melanie S.; Richer, Edward J.; Kudva, Vrinda V.; Paige, Renee C.; Seiler, Michael P.; Liebler, Janice M.; Zabner, Joseph; Plopper, Charles; Brody, Steven L.

In: Journal of Cell Science, Vol. 116, No. 24, 15.12.2003, p. 4935-4945.

Research output: Contribution to journalArticle

Huang, T, You, Y, Spoor, MS, Richer, EJ, Kudva, VV, Paige, RC, Seiler, MP, Liebler, JM, Zabner, J, Plopper, C & Brody, SL 2003, 'Foxj1 is required for apical localization of ezrin in airway epithelial cells', Journal of Cell Science, vol. 116, no. 24, pp. 4935-4945. https://doi.org/10.1242/jcs.00830
Huang T, You Y, Spoor MS, Richer EJ, Kudva VV, Paige RC et al. Foxj1 is required for apical localization of ezrin in airway epithelial cells. Journal of Cell Science. 2003 Dec 15;116(24):4935-4945. https://doi.org/10.1242/jcs.00830
Huang, Tao ; You, You ; Spoor, Melanie S. ; Richer, Edward J. ; Kudva, Vrinda V. ; Paige, Renee C. ; Seiler, Michael P. ; Liebler, Janice M. ; Zabner, Joseph ; Plopper, Charles ; Brody, Steven L. / Foxj1 is required for apical localization of ezrin in airway epithelial cells. In: Journal of Cell Science. 2003 ; Vol. 116, No. 24. pp. 4935-4945.
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