Fos protein expression in the nucleus of the solitary tract in response to intestinal nutrients in awake rats

Tilman T. Zittel; Roberto De Giorgio; Catia Sternini; Helen E Raybould

doi:10.1016/0006-8993(94)91272-6

Fos protein expression in the nucleus of the solitary tract in response to intestinal nutrients in awake rats

Tilman T. Zittel, Roberto De Giorgio, Catia Sternini, Helen E Raybould

School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Nutrients in the intestine inhibit food intake via an action on the vagal afferent pathway. The aim of the present study was to use immunochemical detection of Fos protein-like immunoreactivity (FLI) in the brainstem to trace the neuronal pathways activated by intestinal nutrients. Perfusion of the intestine of awake rats via an indwelling duodenal catheter with iso-osmotic mannitol, hydrochloric acid or casein hydrolysate had no effect on the number of FLI neurons in the nucleus of the solitary tract (NTS). Lipid emulsion (20%) and 2.7 M glucose significantly increased the number of immunopositive cells in the NTS. There was a significant increase in the number of immunopositive cells from caudal to rostral NTS. Nutrients effective at decreasing food intake (carbohydrate and fat) produced significant increases in Fos-like immunopositive cells in the NTS.

Original language	English (US)
Pages (from-to)	266-270
Number of pages	5
Journal	Brain Research
Volume	663
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(94)91272-6
State	Published - Nov 14 1994

Keywords

Carbohydrate
Fos-like immunoreactivity
Immunocytochemistry
Lipid
Nucleus of the solitary tract
Small intestine

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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title = "Fos protein expression in the nucleus of the solitary tract in response to intestinal nutrients in awake rats",

abstract = "Nutrients in the intestine inhibit food intake via an action on the vagal afferent pathway. The aim of the present study was to use immunochemical detection of Fos protein-like immunoreactivity (FLI) in the brainstem to trace the neuronal pathways activated by intestinal nutrients. Perfusion of the intestine of awake rats via an indwelling duodenal catheter with iso-osmotic mannitol, hydrochloric acid or casein hydrolysate had no effect on the number of FLI neurons in the nucleus of the solitary tract (NTS). Lipid emulsion (20%) and 2.7 M glucose significantly increased the number of immunopositive cells in the NTS. There was a significant increase in the number of immunopositive cells from caudal to rostral NTS. Nutrients effective at decreasing food intake (carbohydrate and fat) produced significant increases in Fos-like immunopositive cells in the NTS.",

keywords = "Carbohydrate, Fos-like immunoreactivity, Immunocytochemistry, Lipid, Nucleus of the solitary tract, Small intestine",

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AU - Zittel, Tilman T.

AU - De Giorgio, Roberto

AU - Sternini, Catia

AU - Raybould, Helen E

PY - 1994/11/14

Y1 - 1994/11/14

N2 - Nutrients in the intestine inhibit food intake via an action on the vagal afferent pathway. The aim of the present study was to use immunochemical detection of Fos protein-like immunoreactivity (FLI) in the brainstem to trace the neuronal pathways activated by intestinal nutrients. Perfusion of the intestine of awake rats via an indwelling duodenal catheter with iso-osmotic mannitol, hydrochloric acid or casein hydrolysate had no effect on the number of FLI neurons in the nucleus of the solitary tract (NTS). Lipid emulsion (20%) and 2.7 M glucose significantly increased the number of immunopositive cells in the NTS. There was a significant increase in the number of immunopositive cells from caudal to rostral NTS. Nutrients effective at decreasing food intake (carbohydrate and fat) produced significant increases in Fos-like immunopositive cells in the NTS.

AB - Nutrients in the intestine inhibit food intake via an action on the vagal afferent pathway. The aim of the present study was to use immunochemical detection of Fos protein-like immunoreactivity (FLI) in the brainstem to trace the neuronal pathways activated by intestinal nutrients. Perfusion of the intestine of awake rats via an indwelling duodenal catheter with iso-osmotic mannitol, hydrochloric acid or casein hydrolysate had no effect on the number of FLI neurons in the nucleus of the solitary tract (NTS). Lipid emulsion (20%) and 2.7 M glucose significantly increased the number of immunopositive cells in the NTS. There was a significant increase in the number of immunopositive cells from caudal to rostral NTS. Nutrients effective at decreasing food intake (carbohydrate and fat) produced significant increases in Fos-like immunopositive cells in the NTS.

KW - Carbohydrate

KW - Fos-like immunoreactivity

KW - Immunocytochemistry

KW - Lipid

KW - Nucleus of the solitary tract

KW - Small intestine

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Fos protein expression in the nucleus of the solitary tract in response to intestinal nutrients in awake rats

Abstract

Keywords

ASJC Scopus subject areas

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