Fos protein expression in the nucleus of the solitary tract in response to intestinal nutrients in awake rats

Tilman T. Zittel, Roberto De Giorgio, Catia Sternini, Helen E Raybould

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Nutrients in the intestine inhibit food intake via an action on the vagal afferent pathway. The aim of the present study was to use immunochemical detection of Fos protein-like immunoreactivity (FLI) in the brainstem to trace the neuronal pathways activated by intestinal nutrients. Perfusion of the intestine of awake rats via an indwelling duodenal catheter with iso-osmotic mannitol, hydrochloric acid or casein hydrolysate had no effect on the number of FLI neurons in the nucleus of the solitary tract (NTS). Lipid emulsion (20%) and 2.7 M glucose significantly increased the number of immunopositive cells in the NTS. There was a significant increase in the number of immunopositive cells from caudal to rostral NTS. Nutrients effective at decreasing food intake (carbohydrate and fat) produced significant increases in Fos-like immunopositive cells in the NTS.

Original languageEnglish (US)
Pages (from-to)266-270
Number of pages5
JournalBrain Research
Volume663
Issue number2
DOIs
StatePublished - Nov 14 1994

Fingerprint

Solitary Nucleus
Food
Intestines
Cell Count
Eating
Afferent Pathways
Proteins
Indwelling Catheters
Hydrochloric Acid
Mannitol
Emulsions
Brain Stem
Perfusion
Fats
Carbohydrates
Lipids
Neurons
Glucose

Keywords

  • Carbohydrate
  • Fos-like immunoreactivity
  • Immunocytochemistry
  • Lipid
  • Nucleus of the solitary tract
  • Small intestine

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Fos protein expression in the nucleus of the solitary tract in response to intestinal nutrients in awake rats. / Zittel, Tilman T.; De Giorgio, Roberto; Sternini, Catia; Raybould, Helen E.

In: Brain Research, Vol. 663, No. 2, 14.11.1994, p. 266-270.

Research output: Contribution to journalArticle

Zittel, Tilman T. ; De Giorgio, Roberto ; Sternini, Catia ; Raybould, Helen E. / Fos protein expression in the nucleus of the solitary tract in response to intestinal nutrients in awake rats. In: Brain Research. 1994 ; Vol. 663, No. 2. pp. 266-270.
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