Formula feeding skews immune cell composition toward adaptive immunity compared to breastfeeding

Yvonne Andersson, Marie Louise Hammarström, Bo Lönnerdal, Gitte Graverholt, Helen Fält, Olle Hernell

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The ontogeny of the immune system and the effect thereon by type of infant feeding is incompletely understood. We analyzed frequencies and composition of immune cells in blood of breastfed (BF) and formula-fed (FF) infants at 1.5, 4, and 6 mo of age. Three formulas with the same protein concentration but with varying levels of α-lactalbumin and caseinoglycomacropeptide were compared. Twenty-nine exclusively BF infants served as reference, and 17 infants in each formula group completed the study. Whole blood and PBMCs were analyzed by flow cytometry and immunoflow cytometry, respectively. Leukocyte count of BF infants increased with time due to increased frequency of neutrophils. Lymphocyte count was high at 1.5 mo and was unchanged over time, as were the relative proportions of CD4+ αβT cells, CD8+ αβT cells, B cells, NK cells, and γδT cells. Most CD45R0+CD3+ cells were HLA-DR- and hence memory cells. Compared with breastfeeding, formula feeding resulted in a significant decrease in proportion of NK cells, but a significant increase in naive CD4 + αβT cells and an elevated CD4-to-CD8 ratio, that is, 3.3 in the combined FF groups compared with 2.6 in the BF group. No significant differences were found between the three groups of FF infants. In conclusion, blood cells of lymphoid lineage did not change significantly in frequencies or composition from 1.5 to 6 mo of age in BF infants. In contrast, FF infants displayed an ongoing maturation of adaptive immunity cells and a delayed recruitment of innate immunity cells as compared with BF infants.

Original languageEnglish (US)
Pages (from-to)4322-4328
Number of pages7
JournalJournal of Immunology
Volume183
Issue number7
DOIs
StatePublished - Oct 1 2009

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Adaptive Immunity
Breast Feeding
Infant Formula
T-Lymphocytes
Natural Killer Cells
Blood Cells
Lactalbumin
CD4-CD8 Ratio
Lymphocyte Count
HLA-DR Antigens
Cell Lineage
Leukocyte Count
Innate Immunity
Immune System
Flow Cytometry
Neutrophils
B-Lymphocytes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Andersson, Y., Hammarström, M. L., Lönnerdal, B., Graverholt, G., Fält, H., & Hernell, O. (2009). Formula feeding skews immune cell composition toward adaptive immunity compared to breastfeeding. Journal of Immunology, 183(7), 4322-4328. https://doi.org/10.4049/jimmunol.0900829

Formula feeding skews immune cell composition toward adaptive immunity compared to breastfeeding. / Andersson, Yvonne; Hammarström, Marie Louise; Lönnerdal, Bo; Graverholt, Gitte; Fält, Helen; Hernell, Olle.

In: Journal of Immunology, Vol. 183, No. 7, 01.10.2009, p. 4322-4328.

Research output: Contribution to journalArticle

Andersson, Y, Hammarström, ML, Lönnerdal, B, Graverholt, G, Fält, H & Hernell, O 2009, 'Formula feeding skews immune cell composition toward adaptive immunity compared to breastfeeding', Journal of Immunology, vol. 183, no. 7, pp. 4322-4328. https://doi.org/10.4049/jimmunol.0900829
Andersson Y, Hammarström ML, Lönnerdal B, Graverholt G, Fält H, Hernell O. Formula feeding skews immune cell composition toward adaptive immunity compared to breastfeeding. Journal of Immunology. 2009 Oct 1;183(7):4322-4328. https://doi.org/10.4049/jimmunol.0900829
Andersson, Yvonne ; Hammarström, Marie Louise ; Lönnerdal, Bo ; Graverholt, Gitte ; Fält, Helen ; Hernell, Olle. / Formula feeding skews immune cell composition toward adaptive immunity compared to breastfeeding. In: Journal of Immunology. 2009 ; Vol. 183, No. 7. pp. 4322-4328.
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