Formation of presynaptic terminals at predefined sites along axons

Shasta L. Sabo, Raquel A. Gomes, A Kimberley Usrey

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

What determines where synapses will form along an axon or how proteins are deposited at nascent synapses remains unknown. Here, we show that the initial formation of presynaptic terminals occurs preferentially at predefined sites within the axons of cortical neurons. Time-lapse imaging of synaptic vesicle protein transport vesicles (STVs) indicates that STVs pause repeatedly at these sites, even in the absence of neuronal or glial contact. Contact with a neuroligin-expressing non-neuronal cell induces formation of presynaptic terminals specifically at these STV pause sites. Remarkably, formation of stable contacts with dendritic filopodia also occurs selectively at STV pause sites. Although it is not yet known which molecules comprise the predefined sites, STV pausing is regulated by cues that affect synaptogenesis. Overall, these data are consistent with the hypothesis that regulation of STV pausing might be an important mechanism for accumulation of presynaptic proteins at nascent synapses and support a new model in which many en passant synapses form specifically at predefined sites in young axons.

Original languageEnglish (US)
Pages (from-to)10813-10825
Number of pages13
JournalJournal of Neuroscience
Volume26
Issue number42
DOIs
StatePublished - Oct 18 2006

Keywords

  • Development
  • Synapse formation
  • Synaptogenesis
  • Time-lapse imaging
  • VAMP-EGFP
  • Visual cortex

ASJC Scopus subject areas

  • Neuroscience(all)

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