Sequential moving boundary electrophoresis with protons and hydroxyl ions as the sole counterions, consisting solely of six acids (system A) and of six bases (system B), gives rise to natural steady‐state pH gradients similar to those predicted by theory (pH 2.4–5.5 for system A, pH 10.3–12.2 for system B). Mixtures of the constituents in system A and system B gave rise to a natural pH gradient spanning the pH range between the terminal electrolytes (pH 2.4 and 12.2) predicted for systems A and B. The predicted boundary displacement is negligibly small. Experimentally the boundary displacement could not be determined in view of the difficulty of separating it from the initial transient state in which the pH gradient is formed, and a final state in which the pH gradient decays.
ASJC Scopus subject areas
- Clinical Biochemistry