Formation of an F′ plasmid by recombination between imperfectly repeated chromosomal Rep sequences: A closer look at an old friend (F′128 pro lac)

Eric Kofoid, Ulfar Bergthorsson, E. Susan Slechta, John R. Roth

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Plasmid F′128 was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences. Plasmid F′128 is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification. The structure of F′128 supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac.

Original languageEnglish (US)
Pages (from-to)660-663
Number of pages4
JournalJournal of Bacteriology
Volume185
Issue number2
DOIs
StatePublished - Jan 2003
Externally publishedYes

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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