Abstract
Plasmid F′128 was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences. Plasmid F′128 is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification. The structure of F′128 supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 660-663 |
| Number of pages | 4 |
| Journal | Journal of Bacteriology |
| Volume | 185 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
Formation of an F′ plasmid by recombination between imperfectly repeated chromosomal Rep sequences : A closer look at an old friend (F′128 pro lac). / Kofoid, Eric; Bergthorsson, Ulfar; Slechta, E. Susan; Roth, John R.
In: Journal of Bacteriology, Vol. 185, No. 2, 01.2003, p. 660-663.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Formation of an F′ plasmid by recombination between imperfectly repeated chromosomal Rep sequences
T2 - A closer look at an old friend (F′128 pro lac)
AU - Kofoid, Eric
AU - Bergthorsson, Ulfar
AU - Slechta, E. Susan
AU - Roth, John R.
PY - 2003/1
Y1 - 2003/1
N2 - Plasmid F′128 was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences. Plasmid F′128 is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification. The structure of F′128 supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac.
AB - Plasmid F′128 was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences. Plasmid F′128 is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification. The structure of F′128 supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac.
UR - http://www.scopus.com/inward/record.url?scp=0037207876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037207876&partnerID=8YFLogxK
U2 - 10.1128/JB.185.2.660-663.2003
DO - 10.1128/JB.185.2.660-663.2003
M3 - Article
C2 - 12511513
AN - SCOPUS:0037207876
VL - 185
SP - 660
EP - 663
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 2
ER -