Abstract
Plasmid F′128 was formed by an exchange between chromosomal Rep sequences that placed lac near dinB between many pairs of Rep sequences. Plasmid F′128 is critical for selection-enhanced lac reversion (adaptive mutation), which requires prior lac amplification. The structure of F′128 supports the idea that amplification is initiated by Rep-Rep recombination and that general mutagenesis requires coamplification of dinB (error-prone polymerase) with lac.
Original language | English (US) |
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Pages (from-to) | 660-663 |
Number of pages | 4 |
Journal | Journal of Bacteriology |
Volume | 185 |
Issue number | 2 |
DOIs | |
State | Published - Jan 2003 |
Externally published | Yes |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology