Abstract
Metabolites of arachidonic acid are important regulators of biological function in a variety of mammalian tissues. We have demonstrated similar lipoxygenase enzyme activities in fish gills and mammalian lungs suggesting that their metabolites may have matching functions. Fish gills were investigated for their ability to generate dioxygenated metabolites of polyunsaturated fatty acids. Fatty acids, including arachidonic acid, were incubated with crude tissue homogenates and polar metabolites were extracted, derivatized and analyzed by high performance liquid chromatography, gas chromatography and mass spectrometry. The major dihydroxy metabolite of arachidonic acid was characterized as 8(LR), 15(LS)-dihydroxyeicosatetraenoic acid. This product was formed by the sequential action of the 15- and 12-lipoxygenases in the tissue. The formation of the dihydroxyeicosatetraenoic acid by crude tissue homogenates was significantly enhanced by the addition of 1 mM reduced glutathione. The metabolism of other polyunsaturated fatty acids, including eicosapentaenoic acid and docosahexaenoic acid, to dihydroxy acids was consistent with their relative ability to serve as substrates for the initial 15-lipoxygenase reaction.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 849-853 |
| Number of pages | 5 |
| Journal | Lipids |
| Volume | 25 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1990 |
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ASJC Scopus subject areas
- Medicine (miscellaneous)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Food Science
Cite this
Formation of 8,15-dihydroxy eicosatetraenoic acid via 15- and 12-lipoxygenases in fish gill. / German, J. B.; Berger, Ralf.
In: Lipids, Vol. 25, No. 12, 12.1990, p. 849-853.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Formation of 8,15-dihydroxy eicosatetraenoic acid via 15- and 12-lipoxygenases in fish gill
AU - German, J. B.
AU - Berger, Ralf
PY - 1990/12
Y1 - 1990/12
N2 - Metabolites of arachidonic acid are important regulators of biological function in a variety of mammalian tissues. We have demonstrated similar lipoxygenase enzyme activities in fish gills and mammalian lungs suggesting that their metabolites may have matching functions. Fish gills were investigated for their ability to generate dioxygenated metabolites of polyunsaturated fatty acids. Fatty acids, including arachidonic acid, were incubated with crude tissue homogenates and polar metabolites were extracted, derivatized and analyzed by high performance liquid chromatography, gas chromatography and mass spectrometry. The major dihydroxy metabolite of arachidonic acid was characterized as 8(LR), 15(LS)-dihydroxyeicosatetraenoic acid. This product was formed by the sequential action of the 15- and 12-lipoxygenases in the tissue. The formation of the dihydroxyeicosatetraenoic acid by crude tissue homogenates was significantly enhanced by the addition of 1 mM reduced glutathione. The metabolism of other polyunsaturated fatty acids, including eicosapentaenoic acid and docosahexaenoic acid, to dihydroxy acids was consistent with their relative ability to serve as substrates for the initial 15-lipoxygenase reaction.
AB - Metabolites of arachidonic acid are important regulators of biological function in a variety of mammalian tissues. We have demonstrated similar lipoxygenase enzyme activities in fish gills and mammalian lungs suggesting that their metabolites may have matching functions. Fish gills were investigated for their ability to generate dioxygenated metabolites of polyunsaturated fatty acids. Fatty acids, including arachidonic acid, were incubated with crude tissue homogenates and polar metabolites were extracted, derivatized and analyzed by high performance liquid chromatography, gas chromatography and mass spectrometry. The major dihydroxy metabolite of arachidonic acid was characterized as 8(LR), 15(LS)-dihydroxyeicosatetraenoic acid. This product was formed by the sequential action of the 15- and 12-lipoxygenases in the tissue. The formation of the dihydroxyeicosatetraenoic acid by crude tissue homogenates was significantly enhanced by the addition of 1 mM reduced glutathione. The metabolism of other polyunsaturated fatty acids, including eicosapentaenoic acid and docosahexaenoic acid, to dihydroxy acids was consistent with their relative ability to serve as substrates for the initial 15-lipoxygenase reaction.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025661908&partnerID=8YFLogxK
U2 - 10.1007/BF02535908
DO - 10.1007/BF02535908
M3 - Article
AN - SCOPUS:0025661908
VL - 25
SP - 849
EP - 853
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 12
ER -