Formaldehyde and epigenetic alterations: MicroRNA changes in the nasal epithelium of nonhuman primates

Julia E. Rager, Benjamin Moeller, Melanie Doyle-Eisele, Dean Kracko, James A. Swenberg, Rebecca C. Fry

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Formaldehyde is an air pollutant present in both indoor and outdoor atmospheres. Because of its ubiquitous nature, it is imperative to understand the mechanisms underlying formaldehyde-induced toxicity and carcinogenicity. MicroRNAs (miRNAs) can influence disease caused by environmental exposures, yet miRNAs are understudied in relation to formaldehyde. Our previous investigation demonstrated that formaldehyde exposure in human lung cells caused disruptions in miRNA expression profiles in vitro. Objectives: Using an in vivo model, we set out to test the hypothesis that formaldehyde inhalation exposure significantly alters miRNA expression profiles within the nasal epithelium of nonhuman primates. Methods: Cynomolgus macaques were exposed by inhalation to approximately 0, 2, or 6 ppm formaldehyde for 6 hr/day for 2 consecutive days. Small RNAs were extracted from nasal samples and assessed for genome-wide miRNA expression levels. Transcriptional targets of formaldehyde-altered miRNAs were computationally predicted, analyzed at the systems level, and assessed using real-time reverse transcriptase polymerase chain reaction (RT-PCR). Results: Expression analysis revealed that 3 and 13 miRNAs were dysregulated in response to 2 and 6 ppm formaldehyde, respectively. Transcriptional targets of the miRNA with the greatest increase (miR-125b) and decrease (miR-142-3p) in expression were predicted and analyzed at the systems level. Enrichment was identified for miR-125b targeting genes involved in apoptosis signaling. The apoptosis-related targets were functionally tested using RT-PCR, where all targets showed decreased expression in formaldehyde-exposed samples. Conclusions: Formaldehyde exposure significantly disrupts miRNA expression profiles within the nasal epithelium, and these alterations likely influence apoptosis signaling.

Original languageEnglish (US)
Pages (from-to)339-344
Number of pages6
JournalEnvironmental Health Perspectives
Volume121
Issue number3
DOIs
StatePublished - Mar 11 2013
Externally publishedYes

Fingerprint

Nasal Mucosa
MicroRNAs
Epigenomics
Primates
Formaldehyde
Apoptosis
Reverse Transcriptase Polymerase Chain Reaction
Inhalation Exposure
Air Pollutants
Gene Targeting
Environmental Exposure
Macaca
Atmosphere
Nose
Inhalation
Real-Time Polymerase Chain Reaction
Genome
RNA
Lung
Formaldehyde poisoning

Keywords

  • Apoptosis
  • Epigenetics
  • Formaldehyde
  • MicroRNA
  • Primate
  • Systems biology

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

Formaldehyde and epigenetic alterations : MicroRNA changes in the nasal epithelium of nonhuman primates. / Rager, Julia E.; Moeller, Benjamin; Doyle-Eisele, Melanie; Kracko, Dean; Swenberg, James A.; Fry, Rebecca C.

In: Environmental Health Perspectives, Vol. 121, No. 3, 11.03.2013, p. 339-344.

Research output: Contribution to journalArticle

Rager, Julia E. ; Moeller, Benjamin ; Doyle-Eisele, Melanie ; Kracko, Dean ; Swenberg, James A. ; Fry, Rebecca C. / Formaldehyde and epigenetic alterations : MicroRNA changes in the nasal epithelium of nonhuman primates. In: Environmental Health Perspectives. 2013 ; Vol. 121, No. 3. pp. 339-344.
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