'Form variation' of the O12 antigen is critical for persistence of Salmonella Typhimurium in the murine intestine

Lydia M. Bogomolnaya, Carlos A. Santiviago, Hee Jeong Yang, Andreas J Baumler, Helene L. Andrews-Polymenis

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Salmonella enterica subspecies I serotypes are responsible for the vast majority of salmonellosis in mammals and birds, yet only a few factors specific to this group that allow them to persist in this niche have been identified. We show that STM0557, a S. enterica subspecies I-specific gene encoding an inner membrane protein, is critical for faecal shedding and intestinal persistence of S. enterica serotype Typhimurium ATCC14028 in Salmonella-resistant mice, but mutations in this gene do not diminish short-term intestinal colonization or invasion of cultured epithelial cells. STM0557 and two neighbouring genes, located on a pathogenicity island termed SPI-16, resemble genes of the gtrA,B, gtr(type) cluster in seroconverting bacteriophages. In general, the gtr genes encode proteins responsible for serotype conversion of the infected bacterium by addition glucose residues to repeating O-antigen subunits of lipopolysaccharide (LPS). In lysogenized Shigella, such modifications have been previously shown to be constitutively expressed and to facilitate invasion of host cells. We show that serotype Typhimurium gtr orthologues, STM0557-0559, are responsible for 'form variation' or glucosylation of the O12 antigen galactose (4 position) to generate the 12-2 variant. Form variation in Typhimurium is not constitutive, but occurred upon exposure and during intracellular growth of serotype Typhimurium in J774 macrophages. Our data suggest that the 12-2 antigen is a S. enterica subspecies I-specific LPS modification that enhances long-term intestinal colonization, and is in contrast to the role of O-antigen variation described for Shigella.

Original languageEnglish (US)
Pages (from-to)1105-1119
Number of pages15
JournalMolecular Microbiology
Volume70
Issue number5
DOIs
StatePublished - Dec 2008

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Antigenic Variation
Salmonella typhimurium
Salmonella enterica
Intestines
O Antigens
Shigella
Genes
Lipopolysaccharides
Genomic Islands
Antigens
Salmonella Infections
Galactose
Salmonella
Bacteriophages
Birds
Mammals
Cultured Cells
Membrane Proteins
Epithelial Cells
Macrophages

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

'Form variation' of the O12 antigen is critical for persistence of Salmonella Typhimurium in the murine intestine. / Bogomolnaya, Lydia M.; Santiviago, Carlos A.; Yang, Hee Jeong; Baumler, Andreas J; Andrews-Polymenis, Helene L.

In: Molecular Microbiology, Vol. 70, No. 5, 12.2008, p. 1105-1119.

Research output: Contribution to journalArticle

Bogomolnaya, Lydia M. ; Santiviago, Carlos A. ; Yang, Hee Jeong ; Baumler, Andreas J ; Andrews-Polymenis, Helene L. / 'Form variation' of the O12 antigen is critical for persistence of Salmonella Typhimurium in the murine intestine. In: Molecular Microbiology. 2008 ; Vol. 70, No. 5. pp. 1105-1119.
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