Forced expression of fibroblast growth factor 21 reverses the sustained impairment of liver regeneration in PPARα<sup>PAC</sup> mice due to dysregulated bile acid synthesis

Hui Xin Liu, Ying Hu, Samuel W. French, Frank J. Gonzalez, Yu-Jui Yvonne Wan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Peroxisome proliferator activated receptor α (PPARα) stimulates hepatocellular proliferation is species-specific. Activation of mouse, but not human, PPARα induces hepatocellular proliferation, hepatomegaly, and liver cancer. Here we tested the hypothesis that human and mouse PPARα affects liver regeneration differentially. PPARα-humanized mice (hPPARα<sup>PAC</sup>) were similar to wild type mice in responding to fasting-induced PPARα signaling. However, these mouse livers failed to regenerate in response to partial hepatectomy (PH). The liver-to-body weight ratios did not recover even 3 months after PH in hPPARα<sup>PAC</sup>. The mouse PPARα-mediated down-regulation of let-7c was absent in hPPARα<sup>PAC</sup>, which might partially be responsible for impaired proliferation. After PH, hPPARα<sup>PAC</sup> displayed steatosis, necrosis, and inflammation mainly in periportal zone 1, which suggested bile-induced toxicity. Quantification of hepatic bile acids (BA) revealed BA overload with increased hydrophobic BA in hPPARα<sup>PAC</sup>. Forced FGF21 expression in partial hepatectomized hPPARα<sup>PAC </sup>reduced hepatic steatosis, prevented focal necrosis, and restored liver mass. Compared to mouse PPARα, human PPARα has a reduced capacity to regulate metabolic pathways required for liver regeneration. In addition, FGF21 can compensate for the reduced ability of human PPARα in stimulating liver regeneration, which suggests the potential application of FGF21 in promoting hepatic growth in injured and steatotic livers in humans.

Original languageEnglish (US)
Pages (from-to)9686-9700
Number of pages15
JournalOncotarget
Volume6
Issue number12
StatePublished - 2015

Fingerprint

Peroxisome Proliferator-Activated Receptors
Liver Regeneration
Bile Acids and Salts
Liver
Hepatectomy
Necrosis
fibroblast growth factor 21
Aptitude
Hepatomegaly
Liver Neoplasms
Metabolic Networks and Pathways
Bile
Fasting
Down-Regulation
Body Weight
Inflammation

Keywords

  • Bile acid
  • Metabolism
  • Nuclear receptor
  • Proliferation
  • Species differences

ASJC Scopus subject areas

  • Oncology

Cite this

Forced expression of fibroblast growth factor 21 reverses the sustained impairment of liver regeneration in PPARα<sup>PAC</sup> mice due to dysregulated bile acid synthesis. / Liu, Hui Xin; Hu, Ying; French, Samuel W.; Gonzalez, Frank J.; Wan, Yu-Jui Yvonne.

In: Oncotarget, Vol. 6, No. 12, 2015, p. 9686-9700.

Research output: Contribution to journalArticle

@article{b5c7a28e35304d04acf4cc26ebbe43aa,
title = "Forced expression of fibroblast growth factor 21 reverses the sustained impairment of liver regeneration in PPARαPAC mice due to dysregulated bile acid synthesis",
abstract = "Peroxisome proliferator activated receptor α (PPARα) stimulates hepatocellular proliferation is species-specific. Activation of mouse, but not human, PPARα induces hepatocellular proliferation, hepatomegaly, and liver cancer. Here we tested the hypothesis that human and mouse PPARα affects liver regeneration differentially. PPARα-humanized mice (hPPARαPAC) were similar to wild type mice in responding to fasting-induced PPARα signaling. However, these mouse livers failed to regenerate in response to partial hepatectomy (PH). The liver-to-body weight ratios did not recover even 3 months after PH in hPPARαPAC. The mouse PPARα-mediated down-regulation of let-7c was absent in hPPARαPAC, which might partially be responsible for impaired proliferation. After PH, hPPARαPAC displayed steatosis, necrosis, and inflammation mainly in periportal zone 1, which suggested bile-induced toxicity. Quantification of hepatic bile acids (BA) revealed BA overload with increased hydrophobic BA in hPPARαPAC. Forced FGF21 expression in partial hepatectomized hPPARαPAC reduced hepatic steatosis, prevented focal necrosis, and restored liver mass. Compared to mouse PPARα, human PPARα has a reduced capacity to regulate metabolic pathways required for liver regeneration. In addition, FGF21 can compensate for the reduced ability of human PPARα in stimulating liver regeneration, which suggests the potential application of FGF21 in promoting hepatic growth in injured and steatotic livers in humans.",
keywords = "Bile acid, Metabolism, Nuclear receptor, Proliferation, Species differences",
author = "Liu, {Hui Xin} and Ying Hu and French, {Samuel W.} and Gonzalez, {Frank J.} and Wan, {Yu-Jui Yvonne}",
year = "2015",
language = "English (US)",
volume = "6",
pages = "9686--9700",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "12",

}

TY - JOUR

T1 - Forced expression of fibroblast growth factor 21 reverses the sustained impairment of liver regeneration in PPARαPAC mice due to dysregulated bile acid synthesis

AU - Liu, Hui Xin

AU - Hu, Ying

AU - French, Samuel W.

AU - Gonzalez, Frank J.

AU - Wan, Yu-Jui Yvonne

PY - 2015

Y1 - 2015

N2 - Peroxisome proliferator activated receptor α (PPARα) stimulates hepatocellular proliferation is species-specific. Activation of mouse, but not human, PPARα induces hepatocellular proliferation, hepatomegaly, and liver cancer. Here we tested the hypothesis that human and mouse PPARα affects liver regeneration differentially. PPARα-humanized mice (hPPARαPAC) were similar to wild type mice in responding to fasting-induced PPARα signaling. However, these mouse livers failed to regenerate in response to partial hepatectomy (PH). The liver-to-body weight ratios did not recover even 3 months after PH in hPPARαPAC. The mouse PPARα-mediated down-regulation of let-7c was absent in hPPARαPAC, which might partially be responsible for impaired proliferation. After PH, hPPARαPAC displayed steatosis, necrosis, and inflammation mainly in periportal zone 1, which suggested bile-induced toxicity. Quantification of hepatic bile acids (BA) revealed BA overload with increased hydrophobic BA in hPPARαPAC. Forced FGF21 expression in partial hepatectomized hPPARαPAC reduced hepatic steatosis, prevented focal necrosis, and restored liver mass. Compared to mouse PPARα, human PPARα has a reduced capacity to regulate metabolic pathways required for liver regeneration. In addition, FGF21 can compensate for the reduced ability of human PPARα in stimulating liver regeneration, which suggests the potential application of FGF21 in promoting hepatic growth in injured and steatotic livers in humans.

AB - Peroxisome proliferator activated receptor α (PPARα) stimulates hepatocellular proliferation is species-specific. Activation of mouse, but not human, PPARα induces hepatocellular proliferation, hepatomegaly, and liver cancer. Here we tested the hypothesis that human and mouse PPARα affects liver regeneration differentially. PPARα-humanized mice (hPPARαPAC) were similar to wild type mice in responding to fasting-induced PPARα signaling. However, these mouse livers failed to regenerate in response to partial hepatectomy (PH). The liver-to-body weight ratios did not recover even 3 months after PH in hPPARαPAC. The mouse PPARα-mediated down-regulation of let-7c was absent in hPPARαPAC, which might partially be responsible for impaired proliferation. After PH, hPPARαPAC displayed steatosis, necrosis, and inflammation mainly in periportal zone 1, which suggested bile-induced toxicity. Quantification of hepatic bile acids (BA) revealed BA overload with increased hydrophobic BA in hPPARαPAC. Forced FGF21 expression in partial hepatectomized hPPARαPAC reduced hepatic steatosis, prevented focal necrosis, and restored liver mass. Compared to mouse PPARα, human PPARα has a reduced capacity to regulate metabolic pathways required for liver regeneration. In addition, FGF21 can compensate for the reduced ability of human PPARα in stimulating liver regeneration, which suggests the potential application of FGF21 in promoting hepatic growth in injured and steatotic livers in humans.

KW - Bile acid

KW - Metabolism

KW - Nuclear receptor

KW - Proliferation

KW - Species differences

UR - http://www.scopus.com/inward/record.url?scp=84929590429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929590429&partnerID=8YFLogxK

M3 - Article

C2 - 25991671

AN - SCOPUS:84929590429

VL - 6

SP - 9686

EP - 9700

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 12

ER -