Force versus axial deflection of pipette-aspirated closed membranes

Volkmar Heinrich, Chawin Ounkomol

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The axial deformation of apipette-pressurized fluid membrane bag produces minuscule yet well-defined, reproducible forces. The stiffness of this ultrasensitive force transducer is tunable and largely independent of the constitutive membrane behavior. Based on a rigorous variational treatment, we present both numerical as well as approximate analytical solutions for the force-deflection relation of this unique biophysical force probe. Our numerical results predict a measurably nonlinear force-deflection behavior at moderate-to-large deformations, which we confirm experimentally using red blood cells. Furthermore, considering nearly spherical membrane shapes and enforcing proper boundary conditions, we derive an analytical solution valid at small deformations. In this linear regime the pressurized membrane bag behaves like a Hookean spring, with a spring constant that is significantly larger than previously published for the biomembrane force probe.

Original languageEnglish (US)
Pages (from-to)363-372
Number of pages10
JournalBiophysical Journal
Volume93
Issue number2
DOIs
StatePublished - Jul 2007

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Membranes
Transducers
Erythrocytes

ASJC Scopus subject areas

  • Biophysics

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Force versus axial deflection of pipette-aspirated closed membranes. / Heinrich, Volkmar; Ounkomol, Chawin.

In: Biophysical Journal, Vol. 93, No. 2, 07.2007, p. 363-372.

Research output: Contribution to journalArticle

Heinrich, Volkmar ; Ounkomol, Chawin. / Force versus axial deflection of pipette-aspirated closed membranes. In: Biophysical Journal. 2007 ; Vol. 93, No. 2. pp. 363-372.
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