Abstract
KINESIN is a microtubule-based motor protein that uses energy released from Mg-ATP hydrolysis to generate force for the movement of intracellular membranes towards the fast-growing (plus) ends of microtubule tracks in cells1. Kinesin-driven microtubule movement can be visualized and quantified using light microscope motility assays2-5 but our understanding of how kinesin generates force and motion is incomplete6. Here we report the use of a centrifuge microscope7,8 to obtain force-velocity curves for kinesin-driven motility and to estimate that the maximal isometric force generated per kinesin is 0.12 ± 0.03 pN per molecule.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 457-459 |
| Number of pages | 3 |
| Journal | Nature |
| Volume | 364 |
| Issue number | 6436 |
| State | Published - Jul 29 1993 |
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ASJC Scopus subject areas
- General
Cite this
Force-velocity relationships in kinesin-driven motility. / Hall, Kirsten; Cole, Douglas G.; Yeh, Yin; Scholey, Jonathan M.; Baskin, Ronald J.
In: Nature, Vol. 364, No. 6436, 29.07.1993, p. 457-459.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Force-velocity relationships in kinesin-driven motility
AU - Hall, Kirsten
AU - Cole, Douglas G.
AU - Yeh, Yin
AU - Scholey, Jonathan M.
AU - Baskin, Ronald J.
PY - 1993/7/29
Y1 - 1993/7/29
N2 - KINESIN is a microtubule-based motor protein that uses energy released from Mg-ATP hydrolysis to generate force for the movement of intracellular membranes towards the fast-growing (plus) ends of microtubule tracks in cells1. Kinesin-driven microtubule movement can be visualized and quantified using light microscope motility assays2-5 but our understanding of how kinesin generates force and motion is incomplete6. Here we report the use of a centrifuge microscope7,8 to obtain force-velocity curves for kinesin-driven motility and to estimate that the maximal isometric force generated per kinesin is 0.12 ± 0.03 pN per molecule.
AB - KINESIN is a microtubule-based motor protein that uses energy released from Mg-ATP hydrolysis to generate force for the movement of intracellular membranes towards the fast-growing (plus) ends of microtubule tracks in cells1. Kinesin-driven microtubule movement can be visualized and quantified using light microscope motility assays2-5 but our understanding of how kinesin generates force and motion is incomplete6. Here we report the use of a centrifuge microscope7,8 to obtain force-velocity curves for kinesin-driven motility and to estimate that the maximal isometric force generated per kinesin is 0.12 ± 0.03 pN per molecule.
UR - http://www.scopus.com/inward/record.url?scp=0027224127&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027224127&partnerID=8YFLogxK
M3 - Article
C2 - 8332217
AN - SCOPUS:0027224127
VL - 364
SP - 457
EP - 459
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6436
ER -