Abstract
Single molecule force spectroscopy presents a deceptively simple approach to probing interaction between molecules and molecular assemblies on the nanoscale by measuring forces that it takes to pull the molecules apart. Yet, a more detailed analysis reveals a wealth of different behaviors and interesting physics. This article aims to explore basic physical concepts behind these experiments from a strictly practical point of using these data to extract meaningful information about the interactions. It also focuses on different loading regimes in these experiments, different kinetics that they cause, and different data interpretation that is required for measurements in those regimes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 710-718 |
| Number of pages | 9 |
| Journal | Current Opinion in Chemical Biology |
| Volume | 15 |
| Issue number | 5 |
| DOIs | |
| State | Published - Oct 2011 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
Cite this
Force spectroscopy 101 : How to design, perform, and analyze an AFM-based single molecule force spectroscopy experiment. / Noy, Aleksandr.
In: Current Opinion in Chemical Biology, Vol. 15, No. 5, 10.2011, p. 710-718.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Force spectroscopy 101
T2 - How to design, perform, and analyze an AFM-based single molecule force spectroscopy experiment
AU - Noy, Aleksandr
PY - 2011/10
Y1 - 2011/10
N2 - Single molecule force spectroscopy presents a deceptively simple approach to probing interaction between molecules and molecular assemblies on the nanoscale by measuring forces that it takes to pull the molecules apart. Yet, a more detailed analysis reveals a wealth of different behaviors and interesting physics. This article aims to explore basic physical concepts behind these experiments from a strictly practical point of using these data to extract meaningful information about the interactions. It also focuses on different loading regimes in these experiments, different kinetics that they cause, and different data interpretation that is required for measurements in those regimes.
AB - Single molecule force spectroscopy presents a deceptively simple approach to probing interaction between molecules and molecular assemblies on the nanoscale by measuring forces that it takes to pull the molecules apart. Yet, a more detailed analysis reveals a wealth of different behaviors and interesting physics. This article aims to explore basic physical concepts behind these experiments from a strictly practical point of using these data to extract meaningful information about the interactions. It also focuses on different loading regimes in these experiments, different kinetics that they cause, and different data interpretation that is required for measurements in those regimes.
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U2 - 10.1016/j.cbpa.2011.07.020
DO - 10.1016/j.cbpa.2011.07.020
M3 - Article
VL - 15
SP - 710
EP - 718
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
SN - 1367-5931
IS - 5
ER -