Footprint of the sigma protein: A re-examination

Arthur Wellman; Claude F. Meares

doi:10.1016/0006-291X(91)91959-G

Footprint of the sigma protein: A re-examination

Arthur Wellman, Claude F. Meares

Chemistry

Research output: Contribution to journal › Article

7 Scopus citations

Abstract

Escherichia coli RNA Polymerase is a multi-subunit enzyme that catalyzes RNA synthesis, using DNA as a template. The sigma subunit of this enzyme plays an important role in the recognition of promoter sites on DNA. Using DNase I footprinting, Utpala Ramesh and Claude F. Meares [(1989) Biochem. Biophys. Res. Comm. 160, 121-125] reported that in the absence of the other subunits, sigma binds specifically to the bacteriophage lambda P_R promoter DNA sequence. We are unable to reproduce that result.

Original language	English (US)
Pages (from-to)	140-144
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	177
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(91)91959-G
State	Published - May 31 1991

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Wellman, A., & Meares, C. F. (1991). Footprint of the sigma protein: A re-examination. Biochemical and Biophysical Research Communications, 177(1), 140-144. https://doi.org/10.1016/0006-291X(91)91959-G

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