Footprint of the sigma protein: A re-examination

Arthur Wellman, Claude F. Meares

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Escherichia coli RNA Polymerase is a multi-subunit enzyme that catalyzes RNA synthesis, using DNA as a template. The sigma subunit of this enzyme plays an important role in the recognition of promoter sites on DNA. Using DNase I footprinting, Utpala Ramesh and Claude F. Meares [(1989) Biochem. Biophys. Res. Comm. 160, 121-125] reported that in the absence of the other subunits, sigma binds specifically to the bacteriophage lambda PR promoter DNA sequence. We are unable to reproduce that result.

Original languageEnglish (US)
Pages (from-to)140-144
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume177
Issue number1
DOIs
StatePublished - May 31 1991

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Footprint of the sigma protein: A re-examination'. Together they form a unique fingerprint.

  • Cite this