Footprint of the sigma protein

Utpala Ramesh, Claude F. Meares

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Escherichia coli RNA Polymerase is a multi-subunit enzyme that catalyzes RNA synthesis, using DNA as a template. The sigma subunit of this enzyme plays an important role in the recognition of promoter sites on DNA. Using DNase I footprinting, we have found that in the absence of the other subunits, sigma binds specifically to the bacteriophage lambda PR promoter DNA sequence. In the presence of the sigma subunit alone, a protective footprint encompassing the region between residue positions -41 and +17 was observed (where +1 is the transcription start site). The holoenzyme gave a footprint covering the same region. Thus not only does the sigma subunit interact with the DNA promoter site in the absence of the other components of RNA polymerase, but also the footprint of sigma is indistinguishable from that of the holoenzyme.

Original languageEnglish (US)
Pages (from-to)121-125
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number1
DOIs
StatePublished - Apr 14 1989

Fingerprint

Protein Footprinting
Sigma Factor
Holoenzymes
DNA-Directed RNA Polymerases
DNA
Proteins
Bacteriophage lambda
Bacteriophages
Transcription Initiation Site
Deoxyribonuclease I
DNA sequences
Enzymes
Escherichia coli
RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Footprint of the sigma protein. / Ramesh, Utpala; Meares, Claude F.

In: Biochemical and Biophysical Research Communications, Vol. 160, No. 1, 14.04.1989, p. 121-125.

Research output: Contribution to journalArticle

Ramesh, Utpala ; Meares, Claude F. / Footprint of the sigma protein. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 160, No. 1. pp. 121-125.
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