Food authentication from shotgun sequencing reads with an application on high protein powders

Niina Haiminen, Stefan Edlund, David Chambliss, Mark Kunitomi, Bart C. Weimer, Balasubramanian Ganesan, Robert Baker, Peter Markwell, Matthew Davis, B. Carol Huang, Nguyet Kong, Robert J. Prill, Carl H. Marlowe, André Quintanar, Sophie Pierre, Geraud Dubois, James H. Kaufman, Laxmi Parida, Kristen L. Beck

Research output: Contribution to journalArticle

Abstract

Here we propose that using shotgun sequencing to examine food leads to accurate authentication of ingredients and detection of contaminants. To demonstrate this, we developed a bioinformatic pipeline, FASER (Food Authentication from SEquencing Reads), designed to resolve the relative composition of mixtures of eukaryotic species using RNA or DNA sequencing. Our comprehensive database includes >6000 plants and animals that may be present in food. FASER accurately identified eukaryotic species with 0.4% median absolute difference between observed and expected proportions on sequence data from various sources including sausage meat, plants, and fish. FASER was applied to 31 high protein powder raw factory ingredient total RNA samples. The samples mostly contained the expected source ingredient, chicken, while three samples unexpectedly contained pork and beef. Our results demonstrate that DNA/RNA sequencing of food ingredients, combined with a robust analysis, can be used to find contaminants and authenticate food ingredients in a single assay.

Original languageEnglish (US)
Article number24
Journalnpj Science of Food
Volume3
Issue number1
DOIs
StatePublished - Dec 1 2019

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ASJC Scopus subject areas

  • Food Science
  • Public Health, Environmental and Occupational Health

Cite this

Haiminen, N., Edlund, S., Chambliss, D., Kunitomi, M., Weimer, B. C., Ganesan, B., Baker, R., Markwell, P., Davis, M., Huang, B. C., Kong, N., Prill, R. J., Marlowe, C. H., Quintanar, A., Pierre, S., Dubois, G., Kaufman, J. H., Parida, L., & Beck, K. L. (2019). Food authentication from shotgun sequencing reads with an application on high protein powders. npj Science of Food, 3(1), [24]. https://doi.org/10.1038/s41538-019-0056-6