Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry

Herbert J. Tobias, Maurice E. Pitesky, David P. Fergenson, Paul T. Steele, Joanne Horn, Matthias Frank, Eric E. Gard

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalJournal of Microbiological Methods
Volume67
Issue number1
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

Fingerprint

Bacillus
Mass Spectrometry
Single-Cell Analysis

Keywords

  • Bacillus atrophaeus
  • Endospore formation
  • Single cell mass spectrometry

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry. / Tobias, Herbert J.; Pitesky, Maurice E.; Fergenson, David P.; Steele, Paul T.; Horn, Joanne; Frank, Matthias; Gard, Eric E.

In: Journal of Microbiological Methods, Vol. 67, No. 1, 01.10.2006, p. 56-63.

Research output: Contribution to journalArticle

Tobias, Herbert J. ; Pitesky, Maurice E. ; Fergenson, David P. ; Steele, Paul T. ; Horn, Joanne ; Frank, Matthias ; Gard, Eric E. / Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry. In: Journal of Microbiological Methods. 2006 ; Vol. 67, No. 1. pp. 56-63.
@article{230eba080ec944c5b38a2c0db9bafe93,
title = "Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry",
abstract = "Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.",
keywords = "Bacillus atrophaeus, Endospore formation, Single cell mass spectrometry",
author = "Tobias, {Herbert J.} and Pitesky, {Maurice E.} and Fergenson, {David P.} and Steele, {Paul T.} and Joanne Horn and Matthias Frank and Gard, {Eric E.}",
year = "2006",
month = "10",
day = "1",
doi = "10.1016/j.mimet.2006.03.001",
language = "English (US)",
volume = "67",
pages = "56--63",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry

AU - Tobias, Herbert J.

AU - Pitesky, Maurice E.

AU - Fergenson, David P.

AU - Steele, Paul T.

AU - Horn, Joanne

AU - Frank, Matthias

AU - Gard, Eric E.

PY - 2006/10/1

Y1 - 2006/10/1

N2 - Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.

AB - Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.

KW - Bacillus atrophaeus

KW - Endospore formation

KW - Single cell mass spectrometry

UR - http://www.scopus.com/inward/record.url?scp=33747768985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747768985&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2006.03.001

DO - 10.1016/j.mimet.2006.03.001

M3 - Article

C2 - 16616384

AN - SCOPUS:33747768985

VL - 67

SP - 56

EP - 63

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - 1

ER -