Abstract
Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.
Original language | English (US) |
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Pages (from-to) | 56-63 |
Number of pages | 8 |
Journal | Journal of Microbiological Methods |
Volume | 67 |
Issue number | 1 |
DOIs | |
State | Published - Oct 1 2006 |
Externally published | Yes |
Keywords
- Bacillus atrophaeus
- Endospore formation
- Single cell mass spectrometry
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Microbiology (medical)