Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry

Herbert J. Tobias, Maurice E. Pitesky, David P. Fergenson, Paul T. Steele, Joanne Horn, Matthias Frank, Eric E. Gard

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalJournal of Microbiological Methods
Volume67
Issue number1
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

Keywords

  • Bacillus atrophaeus
  • Endospore formation
  • Single cell mass spectrometry

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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