Abstract
Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 56-63 |
| Number of pages | 8 |
| Journal | Journal of Microbiological Methods |
| Volume | 67 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1 2006 |
| Externally published | Yes |
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Keywords
- Bacillus atrophaeus
- Endospore formation
- Single cell mass spectrometry
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Microbiology (medical)
Cite this
Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry. / Tobias, Herbert J.; Pitesky, Maurice E.; Fergenson, David P.; Steele, Paul T.; Horn, Joanne; Frank, Matthias; Gard, Eric E.
In: Journal of Microbiological Methods, Vol. 67, No. 1, 01.10.2006, p. 56-63.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry
AU - Tobias, Herbert J.
AU - Pitesky, Maurice E.
AU - Fergenson, David P.
AU - Steele, Paul T.
AU - Horn, Joanne
AU - Frank, Matthias
AU - Gard, Eric E.
PY - 2006/10/1
Y1 - 2006/10/1
N2 - Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.
AB - Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.
KW - Bacillus atrophaeus
KW - Endospore formation
KW - Single cell mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=33747768985&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33747768985&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2006.03.001
DO - 10.1016/j.mimet.2006.03.001
M3 - Article
C2 - 16616384
AN - SCOPUS:33747768985
VL - 67
SP - 56
EP - 63
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
IS - 1
ER -