Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry

Herbert J. Tobias; Maurice E. Pitesky; David P. Fergenson; Paul T. Steele; Joanne Horn; Matthias Frank; Eric E. Gard

doi:10.1016/j.mimet.2006.03.001

Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry

Herbert J. Tobias, Maurice E. Pitesky, David P. Fergenson, Paul T. Steele, Joanne Horn, Matthias Frank, Eric E. Gard

Research output: Contribution to journal › Article

19 Scopus citations

Abstract

Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.

Original language	English (US)
Pages (from-to)	56-63
Number of pages	8
Journal	Journal of Microbiological Methods
Volume	67
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2006.03.001
State	Published - Oct 1 2006
Externally published	Yes

Keywords

Bacillus atrophaeus
Endospore formation
Single cell mass spectrometry

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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Tobias, H. J., Pitesky, M. E., Fergenson, D. P., Steele, P. T., Horn, J., Frank, M., & Gard, E. E. (2006). Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry. Journal of Microbiological Methods, 67(1), 56-63. https://doi.org/10.1016/j.mimet.2006.03.001

Following the biochemical and morphological changes of Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry. / Tobias, Herbert J.; Pitesky, Maurice E.; Fergenson, David P.; Steele, Paul T.; Horn, Joanne; Frank, Matthias; Gard, Eric E.

In: Journal of Microbiological Methods, Vol. 67, No. 1, 01.10.2006, p. 56-63.

Research output: Contribution to journal › Article

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abstract = "Bioaerosol Mass Spectrometry (BAMS), a real-time single cell analytical technique, was used to follow the biochemical and morphological changes within a group of Bacillus atrophaeus cells by measuring individual cells during the process of sporulation. A mutant of B. atrophaeus that lacks the ability to produce dipicolinic acid (DPA) was also analyzed. Single cell aerodynamic sizing was used to follow gross morphological changes, and chemical analysis of single cells by mass spectrometry was used to follow some biochemical changes of B. atrophaeus cells during endospore formation.",

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AU - Horn, Joanne

AU - Frank, Matthias

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