Folate deficiency in rats induces DNA strand breaks and hypomethylation within the p53 tumor suppresser gene

Young In Kim, Igor P. Pogribny, Alexei G. Basnakian, Joshua W. Miller, Jacob Selhub, S. Jill James, Joel B. Mason

Research output: Contribution to journalArticle

265 Scopus citations

Abstract

Folate is essential for the de hove biosynthesis of purines and thymidylate, and is an important mediator in the transfer of methyl groups for DNA methylation. Folate deficiency, therefore, could contribute to abnormal DNA integrity and methylation patterns. We investigated the effect of isolated folate deficiency in rats on DNA methylation and DNA strand breaks both at the genomic level and within specific sequences of the p53 tumor suppressor gene. Our data indicate that relate deficiency induces DNA strand breaks and hypomethylation within the p53 gene. Such alterations either did not occur or were chronologically delayed when examined on a genome-wide basis, indicating some selectivity for the exons examined within the p53 gene. Folate insufficiency has been implicated in the development of several human and experimental cancers, and aberrations within these regions of the p53 gene that were examined in this study are thought to play an integral role in carcinogenesis. The aforementioned molecular alterations may therefore be a means by which dietary folate deficiency enhances carcinogenesis.

Original languageEnglish (US)
Pages (from-to)46-52
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume65
Issue number1
StatePublished - 1997
Externally publishedYes

    Fingerprint

Keywords

  • carcinogenesis
  • DNA damage
  • DNA methylation
  • Folate
  • p53

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Kim, Y. I., Pogribny, I. P., Basnakian, A. G., Miller, J. W., Selhub, J., James, S. J., & Mason, J. B. (1997). Folate deficiency in rats induces DNA strand breaks and hypomethylation within the p53 tumor suppresser gene. American Journal of Clinical Nutrition, 65(1), 46-52.