Focal brain injury induces multiple immediate early genes encoding zinc finger transcription factors

Jari Honkaniemi, Stephen M. Sagar, Ilkka Pyykönen, Kathleen J. Hicks, Frank R Sharp

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Focal brain injury is known to markedly induce the fos and jun families of immediate early genes (IEGs). This study employed in situ hybridization to examine the effects of focal brain injury, produced by the intracerebral injection of saline or ibotenic acid on the expression of IEGs encoding zinc finger transcription factors. Thirty minutes after the injections, NGFI-A, NGFI-B, NGFI-C, and egr-3 mRNAs were induced in dentate gyrus, hippocampal pyramidal cells, cerebral cortex, caudate-putamen and piriform cortex of the injured hemisphere. Nurr1 was induced in hippocampal pyramidal cells and dentate granule cells. After three hours the induction of NGFI-A, NGFI-B, NGFI-C and Nurr1 persisted in all brain regions except for the dentate granule cells. By six hours after injection mRNAs for most of the zinc finger genes had returned to control levels. However, the expression of egr-3 3 and 6 h after the injection was identical to that observed at 30 min after the injection and it was the only gene the expression of which persisted 6 h following the injections. Twenty-four hours after the injection, the expression of all five IEGs returned to control levels. In general, no gross differences in the IEG induction were observed between the animals injected with saline and ibotenic acid. Since these zinc finger genes were expressed in the same regions where fos and jun family members are induced by similar types of brain injury, it is suggested that these transcription factors may act in concert with Fos/Jun family members. These observations should be considered when studying the effect of invasive manipulations on IEG expression in the brain and add to the evidence for remote effects of focal brain injury.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalMolecular Brain Research
Volume28
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Immediate-Early Genes
Zinc Fingers
Brain Injuries
Transcription Factors
Injections
Ibotenic Acid
Pyramidal Cells
Gene Expression
Messenger RNA
Putamen
Dentate Gyrus
Brain
Cerebral Cortex
Genes
In Situ Hybridization

Keywords

  • egr-3
  • Hippocampus
  • mRNA
  • NGFI-A
  • NGFI-B
  • NGFI-C
  • Nurr1
  • Spreading depression

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Focal brain injury induces multiple immediate early genes encoding zinc finger transcription factors. / Honkaniemi, Jari; Sagar, Stephen M.; Pyykönen, Ilkka; Hicks, Kathleen J.; Sharp, Frank R.

In: Molecular Brain Research, Vol. 28, No. 1, 1995, p. 157-163.

Research output: Contribution to journalArticle

Honkaniemi, Jari ; Sagar, Stephen M. ; Pyykönen, Ilkka ; Hicks, Kathleen J. ; Sharp, Frank R. / Focal brain injury induces multiple immediate early genes encoding zinc finger transcription factors. In: Molecular Brain Research. 1995 ; Vol. 28, No. 1. pp. 157-163.
@article{dd65cc5228d34b8da386bb86c08757de,
title = "Focal brain injury induces multiple immediate early genes encoding zinc finger transcription factors",
abstract = "Focal brain injury is known to markedly induce the fos and jun families of immediate early genes (IEGs). This study employed in situ hybridization to examine the effects of focal brain injury, produced by the intracerebral injection of saline or ibotenic acid on the expression of IEGs encoding zinc finger transcription factors. Thirty minutes after the injections, NGFI-A, NGFI-B, NGFI-C, and egr-3 mRNAs were induced in dentate gyrus, hippocampal pyramidal cells, cerebral cortex, caudate-putamen and piriform cortex of the injured hemisphere. Nurr1 was induced in hippocampal pyramidal cells and dentate granule cells. After three hours the induction of NGFI-A, NGFI-B, NGFI-C and Nurr1 persisted in all brain regions except for the dentate granule cells. By six hours after injection mRNAs for most of the zinc finger genes had returned to control levels. However, the expression of egr-3 3 and 6 h after the injection was identical to that observed at 30 min after the injection and it was the only gene the expression of which persisted 6 h following the injections. Twenty-four hours after the injection, the expression of all five IEGs returned to control levels. In general, no gross differences in the IEG induction were observed between the animals injected with saline and ibotenic acid. Since these zinc finger genes were expressed in the same regions where fos and jun family members are induced by similar types of brain injury, it is suggested that these transcription factors may act in concert with Fos/Jun family members. These observations should be considered when studying the effect of invasive manipulations on IEG expression in the brain and add to the evidence for remote effects of focal brain injury.",
keywords = "egr-3, Hippocampus, mRNA, NGFI-A, NGFI-B, NGFI-C, Nurr1, Spreading depression",
author = "Jari Honkaniemi and Sagar, {Stephen M.} and Ilkka Pyyk{\"o}nen and Hicks, {Kathleen J.} and Sharp, {Frank R}",
year = "1995",
doi = "10.1016/0169-328X(94)00200-X",
language = "English (US)",
volume = "28",
pages = "157--163",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Focal brain injury induces multiple immediate early genes encoding zinc finger transcription factors

AU - Honkaniemi, Jari

AU - Sagar, Stephen M.

AU - Pyykönen, Ilkka

AU - Hicks, Kathleen J.

AU - Sharp, Frank R

PY - 1995

Y1 - 1995

N2 - Focal brain injury is known to markedly induce the fos and jun families of immediate early genes (IEGs). This study employed in situ hybridization to examine the effects of focal brain injury, produced by the intracerebral injection of saline or ibotenic acid on the expression of IEGs encoding zinc finger transcription factors. Thirty minutes after the injections, NGFI-A, NGFI-B, NGFI-C, and egr-3 mRNAs were induced in dentate gyrus, hippocampal pyramidal cells, cerebral cortex, caudate-putamen and piriform cortex of the injured hemisphere. Nurr1 was induced in hippocampal pyramidal cells and dentate granule cells. After three hours the induction of NGFI-A, NGFI-B, NGFI-C and Nurr1 persisted in all brain regions except for the dentate granule cells. By six hours after injection mRNAs for most of the zinc finger genes had returned to control levels. However, the expression of egr-3 3 and 6 h after the injection was identical to that observed at 30 min after the injection and it was the only gene the expression of which persisted 6 h following the injections. Twenty-four hours after the injection, the expression of all five IEGs returned to control levels. In general, no gross differences in the IEG induction were observed between the animals injected with saline and ibotenic acid. Since these zinc finger genes were expressed in the same regions where fos and jun family members are induced by similar types of brain injury, it is suggested that these transcription factors may act in concert with Fos/Jun family members. These observations should be considered when studying the effect of invasive manipulations on IEG expression in the brain and add to the evidence for remote effects of focal brain injury.

AB - Focal brain injury is known to markedly induce the fos and jun families of immediate early genes (IEGs). This study employed in situ hybridization to examine the effects of focal brain injury, produced by the intracerebral injection of saline or ibotenic acid on the expression of IEGs encoding zinc finger transcription factors. Thirty minutes after the injections, NGFI-A, NGFI-B, NGFI-C, and egr-3 mRNAs were induced in dentate gyrus, hippocampal pyramidal cells, cerebral cortex, caudate-putamen and piriform cortex of the injured hemisphere. Nurr1 was induced in hippocampal pyramidal cells and dentate granule cells. After three hours the induction of NGFI-A, NGFI-B, NGFI-C and Nurr1 persisted in all brain regions except for the dentate granule cells. By six hours after injection mRNAs for most of the zinc finger genes had returned to control levels. However, the expression of egr-3 3 and 6 h after the injection was identical to that observed at 30 min after the injection and it was the only gene the expression of which persisted 6 h following the injections. Twenty-four hours after the injection, the expression of all five IEGs returned to control levels. In general, no gross differences in the IEG induction were observed between the animals injected with saline and ibotenic acid. Since these zinc finger genes were expressed in the same regions where fos and jun family members are induced by similar types of brain injury, it is suggested that these transcription factors may act in concert with Fos/Jun family members. These observations should be considered when studying the effect of invasive manipulations on IEG expression in the brain and add to the evidence for remote effects of focal brain injury.

KW - egr-3

KW - Hippocampus

KW - mRNA

KW - NGFI-A

KW - NGFI-B

KW - NGFI-C

KW - Nurr1

KW - Spreading depression

UR - http://www.scopus.com/inward/record.url?scp=0028887868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028887868&partnerID=8YFLogxK

U2 - 10.1016/0169-328X(94)00200-X

DO - 10.1016/0169-328X(94)00200-X

M3 - Article

C2 - 7707870

AN - SCOPUS:0028887868

VL - 28

SP - 157

EP - 163

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -