Fluorescent in situ hybridization assessment of chromosome 8 copy number in breast cancer

Alaa M Afify, Kirby I. Bland, Hon Fong Louie Mark

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Conventional cytogenetics of breast and other solid tumors has been hampered by a number of factors. An analysis of breast tumor tissues was therefore undertaken using fluorescent in situ hybridization (FISH). A total of 34 specimens were analyzed using a chromosome 8-specific α-satellite probe. Various approaches were tested and compared. Among 30 informative samples, 11 infiltrating ductal carcinomas, not otherwise specified (NOS), 5 ductal carcinomas in situ, 5 lobular carcinomas, 3 papillary carcinomas, and 6 benign lesions were studied. Of the 11 cases of infiltrating ductal carcinomas (NOS) analyzed, four cases showed 3 signals, one case showed 4 signals, and the rest showed 2 signals. Of the 5 cases of ductal carcinoma in situ samples, 1 showed 3 signals and the other 4 cases showed 2 signals. All cases of lobular carcinomas, papillary carcinomas, and benign lesions showed 2 signals. We inferred from these data that 36% of the infiltrating ductal carcinomas (NOS) were trisomic and 9% were tetrasomic, whereas 20% of the ductal carcinomas in situ were trisomic. All samples from lobular carcinomas, papillary carcinomas, and the benign lesions were disomic. From our preliminary data, it can further be concluded that a subset of breast cancer is characterized by chromosome 8 trisomy. These data are consistent with an ever-increasing database on the association of chromosomal 8 trisomy with other cancers such as leukemia, lymphoma, prostate cancer, ovarian carcinoma, salivary gland tumor, malignant melanoma, desmoid tumors, and recently gestational trophoblastic disease. It is also noted that the ability to analyze formalin-fixed, paraffin-embedded archival material will enable a more comprehensive cytogenetic study of breast cancer than is currently available.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalBreast Cancer Research and Treatment
Volume38
Issue number2
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Lobular Carcinoma
Chromosomes, Human, Pair 8
Ductal Carcinoma
Carcinoma, Intraductal, Noninfiltrating
Papillary Carcinoma
Fluorescence In Situ Hybridization
Breast Neoplasms
Cytogenetics
Gestational Trophoblastic Disease
Aggressive Fibromatosis
Neoplasms
Glandular and Epithelial Neoplasms
Paraffin
Formaldehyde
Melanoma
Lymphoma
Prostatic Neoplasms
Leukemia
Breast
Databases

Keywords

  • Breast cancer
  • Cytogenetics
  • FISH
  • Fluorescent in situ hybridization
  • Histopathology

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Fluorescent in situ hybridization assessment of chromosome 8 copy number in breast cancer. / Afify, Alaa M; Bland, Kirby I.; Mark, Hon Fong Louie.

In: Breast Cancer Research and Treatment, Vol. 38, No. 2, 1996, p. 201-208.

Research output: Contribution to journalArticle

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abstract = "Conventional cytogenetics of breast and other solid tumors has been hampered by a number of factors. An analysis of breast tumor tissues was therefore undertaken using fluorescent in situ hybridization (FISH). A total of 34 specimens were analyzed using a chromosome 8-specific α-satellite probe. Various approaches were tested and compared. Among 30 informative samples, 11 infiltrating ductal carcinomas, not otherwise specified (NOS), 5 ductal carcinomas in situ, 5 lobular carcinomas, 3 papillary carcinomas, and 6 benign lesions were studied. Of the 11 cases of infiltrating ductal carcinomas (NOS) analyzed, four cases showed 3 signals, one case showed 4 signals, and the rest showed 2 signals. Of the 5 cases of ductal carcinoma in situ samples, 1 showed 3 signals and the other 4 cases showed 2 signals. All cases of lobular carcinomas, papillary carcinomas, and benign lesions showed 2 signals. We inferred from these data that 36{\%} of the infiltrating ductal carcinomas (NOS) were trisomic and 9{\%} were tetrasomic, whereas 20{\%} of the ductal carcinomas in situ were trisomic. All samples from lobular carcinomas, papillary carcinomas, and the benign lesions were disomic. From our preliminary data, it can further be concluded that a subset of breast cancer is characterized by chromosome 8 trisomy. These data are consistent with an ever-increasing database on the association of chromosomal 8 trisomy with other cancers such as leukemia, lymphoma, prostate cancer, ovarian carcinoma, salivary gland tumor, malignant melanoma, desmoid tumors, and recently gestational trophoblastic disease. It is also noted that the ability to analyze formalin-fixed, paraffin-embedded archival material will enable a more comprehensive cytogenetic study of breast cancer than is currently available.",
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