Abstract
Bio-analytical techniques that utitize fluorescence resonance energy transfer (FRET) are particularly attractive to scientists due to the intrinsic sensitivity of this photo-physical process for detecting small variations in molecular distance and orientation. This means, when appropriately configured, any change in proximity between 2 appropriately Labeled molecules can be detected both qualitatively and quantitatively. FRET is finding an ever increasing role in bio-analytical techniques, especially in the monitoring of binding events both intra-cellularly and as a reporter for in vitro assays. Furthermore, use of FRET can dramatically improve optical resolution in multi-analyte or'multiplex'roles. In the present review, we describe many aspects of FRET as an analytical technique with a special emphasis on items of interest to the biologist in general. Among the areas covered are a brief history of FRET, an introduction to relevant concepts and Föster formalism, the mechanism of FRET, FRET as a tool to elucidate biological structures, FRET as a sensing technique in vitro and in vivo, FRET usage with analytical and diagnostic DNA testing, as well as the use of novel proteins and nanocrystalline quantum dot materials in FRET. As more researchers learn about this versatile technique, FRET will continue to gain an ever increasing role in bio-applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 253-279 |
| Number of pages | 27 |
| Journal | Minerva Biotecnologica |
| Volume | 16 |
| Issue number | 4 |
| State | Published - Dec 2004 |
| Externally published | Yes |
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Keywords
- Biotechnology
- Cellular imaging
- Fluorescence resonance energy transfer
- Molecular biology
- Quantum dot
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Fluorescence resonance energy transfer : Concepts, applications and advances. / Sapsford, K. E.; Berti, L.; Medintz, I. L.
In: Minerva Biotecnologica, Vol. 16, No. 4, 12.2004, p. 253-279.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fluorescence resonance energy transfer
T2 - Concepts, applications and advances
AU - Sapsford, K. E.
AU - Berti, L.
AU - Medintz, I. L.
PY - 2004/12
Y1 - 2004/12
N2 - Bio-analytical techniques that utitize fluorescence resonance energy transfer (FRET) are particularly attractive to scientists due to the intrinsic sensitivity of this photo-physical process for detecting small variations in molecular distance and orientation. This means, when appropriately configured, any change in proximity between 2 appropriately Labeled molecules can be detected both qualitatively and quantitatively. FRET is finding an ever increasing role in bio-analytical techniques, especially in the monitoring of binding events both intra-cellularly and as a reporter for in vitro assays. Furthermore, use of FRET can dramatically improve optical resolution in multi-analyte or'multiplex'roles. In the present review, we describe many aspects of FRET as an analytical technique with a special emphasis on items of interest to the biologist in general. Among the areas covered are a brief history of FRET, an introduction to relevant concepts and Föster formalism, the mechanism of FRET, FRET as a tool to elucidate biological structures, FRET as a sensing technique in vitro and in vivo, FRET usage with analytical and diagnostic DNA testing, as well as the use of novel proteins and nanocrystalline quantum dot materials in FRET. As more researchers learn about this versatile technique, FRET will continue to gain an ever increasing role in bio-applications.
AB - Bio-analytical techniques that utitize fluorescence resonance energy transfer (FRET) are particularly attractive to scientists due to the intrinsic sensitivity of this photo-physical process for detecting small variations in molecular distance and orientation. This means, when appropriately configured, any change in proximity between 2 appropriately Labeled molecules can be detected both qualitatively and quantitatively. FRET is finding an ever increasing role in bio-analytical techniques, especially in the monitoring of binding events both intra-cellularly and as a reporter for in vitro assays. Furthermore, use of FRET can dramatically improve optical resolution in multi-analyte or'multiplex'roles. In the present review, we describe many aspects of FRET as an analytical technique with a special emphasis on items of interest to the biologist in general. Among the areas covered are a brief history of FRET, an introduction to relevant concepts and Föster formalism, the mechanism of FRET, FRET as a tool to elucidate biological structures, FRET as a sensing technique in vitro and in vivo, FRET usage with analytical and diagnostic DNA testing, as well as the use of novel proteins and nanocrystalline quantum dot materials in FRET. As more researchers learn about this versatile technique, FRET will continue to gain an ever increasing role in bio-applications.
KW - Biotechnology
KW - Cellular imaging
KW - Fluorescence resonance energy transfer
KW - Molecular biology
KW - Quantum dot
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M3 - Article
VL - 16
SP - 253
EP - 279
JO - Minerva Biotecnologica
JF - Minerva Biotecnologica
SN - 1120-4826
IS - 4
ER -