Fluorescence microscopy imaging of electroperturbation in mammalian cells

Yinghua Sun, P. Thomas Vernier, Matthew Behrend, Jingjing Wang, Mya Mya Thu, Martin Gundersen, Laura Marcu

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

We report the design, integration, and validation of a fluorescence microscopy system for imaging of electroperturbation-the effects of nanosecond, megavolt-per-meter pulsed electric fields on biological cells and tissues. Such effects have potential applications in cancer therapy, gene regulation, and biophysical research by noninvasively disrupting intracellular compartments and inducing apoptosis in malignant cells. As the primary observing platform, an epifluorescence microscope integrating a nanosecond high-voltage pulser and a micrometer electrode chamber enable in situ imaging of the intracellular processes triggered by high electric fields. Using specific fluorescence molecular probes, the dynamic biological responses of Jurkat T lymphocytes to nanosecond electric pulses (nanoelectropulses) are studied with this system, including calcium bursts, the polarized translocation of phosphatidylserine (PS), and nuclear enlargement and chromatin/DNA structural changes.

Original languageEnglish (US)
Article number024010
JournalJournal of Biomedical Optics
Volume11
Issue number2
DOIs
StatePublished - Mar 2006

Keywords

  • Calcium bursts
  • Electroperturbation
  • Electroporation
  • Fluorescence microscopy imaging
  • Phospholipid translocation
  • Pulsed electric field

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

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  • Cite this

    Sun, Y., Thomas Vernier, P., Behrend, M., Wang, J., Thu, M. M., Gundersen, M., & Marcu, L. (2006). Fluorescence microscopy imaging of electroperturbation in mammalian cells. Journal of Biomedical Optics, 11(2), [024010]. https://doi.org/10.1117/1.2187970