Fluorescence lifetime techniques in medical applications

Laura Marcu

Research output: Contribution to journalArticlepeer-review

140 Scopus citations


This article presents an overview of time-resolved (lifetime) fluorescence techniques used in biomedical diagnostics. In particular, we review the development of time-resolved fluorescence spectroscopy (TRFS) and fluorescence lifetime imaging (FLIM) instrumentation and associated methodologies which allow in vivo characterization and diagnosis of biological tissues. Emphasis is placed on the translational research potential of these techniques and on evaluating whether intrinsic fluorescence signals provide useful contrast for the diagnosis of human diseases including cancer (gastrointestinal tract, lung, head and neck, and brain), skin and eye diseases, and atherosclerotic cardiovascular disease.

Original languageEnglish (US)
Pages (from-to)304-331
Number of pages28
JournalAnnals of Biomedical Engineering
Issue number2
StatePublished - Feb 2012


  • Medical applications of optical technologies
  • Time-resolved fluorescence spectroscopy and imaging
  • Tissue diagnosis in vivo

ASJC Scopus subject areas

  • Biomedical Engineering


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