Abstract
During breast conserving surgery (BCS), which is the preferred approach to treat most early stage breast cancers, the surgeon attempts to excise the tumor volume, surrounded by thin margin of normal tissue. The intra-operative assessment of cancerous areas is a challenging procedure, with the surgeon usually relying on visual or tactile guidance. This study evaluates whether time-resolved fluorescence spectroscopy (TRFS) presents the potential to address this problem. Point TRFS measurements were obtained from 19 fresh tissue slices (7 patients) and parameters that characterize the transient signals were quantified via constrained least squares deconvolution scheme. Fibrotic tissue (FT, n=69), adipose tissue (AT, n=76), and invasive ductal carcinoma (IDC, n=27) were identified in histology and univariate statistical analysis, followed by multi-comparison test, was applied to the corresponding lifetime data. Significant differentiation between the three tissue types exists at 390 nm and 500 nm bands. The average lifetime is 3.23±0.74 ns for AT, 4.21±0.83 ns for FT and 4.71±0.35 ns (p<0.05) for IDC at 390 nm. Due to the smaller contribution of collagen in AT the average lifetime value is different from FT and IDC. Additionally, although intensity measurements do not show difference between FT and IDC, lifetime can distinguish them. Similarly, in 500 nm these values are 7.01±1.08 ns, 5.43±1.05 ns and 4.39±0.88 ns correspondingly (p<0.05) and this contrast is due to differentiation in retinol or flavins relative concentration, mostly contributing to AT. Results demonstrate the potential of TRFS to intra-operatively characterize BCS breast excised tissue in real-time and assess tumor margins.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Publisher | SPIE |
| Volume | 9318 |
| ISBN (Print) | 9781628414080 |
| DOIs | |
| State | Published - 2015 |
| Event | Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis - San Francisco, United States Duration: Feb 10 2015 → Feb 11 2015 |
Other
| Other | Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 2/10/15 → 2/11/15 |
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Keywords
- Breast cancer
- Lifetime
- Time-resolved fluorescence spectroscopy
- Tumor margins
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Fluorescence lifetime spectroscopy for breast cancer margins assessment. / Gorpas, Dimitris; Fatakdawala, Hussain; Zhang, Yanhong; Bold, Richard J; Marcu, Laura.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9318 SPIE, 2015. 93180B.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Fluorescence lifetime spectroscopy for breast cancer margins assessment
AU - Gorpas, Dimitris
AU - Fatakdawala, Hussain
AU - Zhang, Yanhong
AU - Bold, Richard J
AU - Marcu, Laura
PY - 2015
Y1 - 2015
N2 - During breast conserving surgery (BCS), which is the preferred approach to treat most early stage breast cancers, the surgeon attempts to excise the tumor volume, surrounded by thin margin of normal tissue. The intra-operative assessment of cancerous areas is a challenging procedure, with the surgeon usually relying on visual or tactile guidance. This study evaluates whether time-resolved fluorescence spectroscopy (TRFS) presents the potential to address this problem. Point TRFS measurements were obtained from 19 fresh tissue slices (7 patients) and parameters that characterize the transient signals were quantified via constrained least squares deconvolution scheme. Fibrotic tissue (FT, n=69), adipose tissue (AT, n=76), and invasive ductal carcinoma (IDC, n=27) were identified in histology and univariate statistical analysis, followed by multi-comparison test, was applied to the corresponding lifetime data. Significant differentiation between the three tissue types exists at 390 nm and 500 nm bands. The average lifetime is 3.23±0.74 ns for AT, 4.21±0.83 ns for FT and 4.71±0.35 ns (p<0.05) for IDC at 390 nm. Due to the smaller contribution of collagen in AT the average lifetime value is different from FT and IDC. Additionally, although intensity measurements do not show difference between FT and IDC, lifetime can distinguish them. Similarly, in 500 nm these values are 7.01±1.08 ns, 5.43±1.05 ns and 4.39±0.88 ns correspondingly (p<0.05) and this contrast is due to differentiation in retinol or flavins relative concentration, mostly contributing to AT. Results demonstrate the potential of TRFS to intra-operatively characterize BCS breast excised tissue in real-time and assess tumor margins.
AB - During breast conserving surgery (BCS), which is the preferred approach to treat most early stage breast cancers, the surgeon attempts to excise the tumor volume, surrounded by thin margin of normal tissue. The intra-operative assessment of cancerous areas is a challenging procedure, with the surgeon usually relying on visual or tactile guidance. This study evaluates whether time-resolved fluorescence spectroscopy (TRFS) presents the potential to address this problem. Point TRFS measurements were obtained from 19 fresh tissue slices (7 patients) and parameters that characterize the transient signals were quantified via constrained least squares deconvolution scheme. Fibrotic tissue (FT, n=69), adipose tissue (AT, n=76), and invasive ductal carcinoma (IDC, n=27) were identified in histology and univariate statistical analysis, followed by multi-comparison test, was applied to the corresponding lifetime data. Significant differentiation between the three tissue types exists at 390 nm and 500 nm bands. The average lifetime is 3.23±0.74 ns for AT, 4.21±0.83 ns for FT and 4.71±0.35 ns (p<0.05) for IDC at 390 nm. Due to the smaller contribution of collagen in AT the average lifetime value is different from FT and IDC. Additionally, although intensity measurements do not show difference between FT and IDC, lifetime can distinguish them. Similarly, in 500 nm these values are 7.01±1.08 ns, 5.43±1.05 ns and 4.39±0.88 ns correspondingly (p<0.05) and this contrast is due to differentiation in retinol or flavins relative concentration, mostly contributing to AT. Results demonstrate the potential of TRFS to intra-operatively characterize BCS breast excised tissue in real-time and assess tumor margins.
KW - Breast cancer
KW - Lifetime
KW - Time-resolved fluorescence spectroscopy
KW - Tumor margins
UR - http://www.scopus.com/inward/record.url?scp=84928547612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84928547612&partnerID=8YFLogxK
U2 - 10.1117/12.2079622
DO - 10.1117/12.2079622
M3 - Conference contribution
AN - SCOPUS:84928547612
SN - 9781628414080
VL - 9318
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
PB - SPIE
ER -