Fluorescence lifetime imaging microscopy: In vivo application to diagnosis of oral carcinoma

Yinghua Sun, Jennifer Phipps, Daniel S. Elson, Heather Stoy, Steven Tinling, Jeremy Meier, Brian Poirier, Frank Chuang, D Gregory Farwell, Laura Marcu

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

A compact clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was designed and built for intraoperative disease diagnosis and validated in vivo in a hamster oral carcinogenesis model. This apparatus allows for the remote image collection via a flexible imaging probe consisting of a gradient index objective lens and a fiber bundle. Tissue autofluorescence (337 nm excitation) was imaged using an intensified CCD with a gate width down to 0.2 ns. We demonstrate a significant contrast in fluorescence lifetime between tumor (1.77±0.26 ns) and normal (2.50±0.36 ns) tissues at 450 nm and an over 80% intensity decrease at 390 nm emission in tumor versus normal areas. The time-resolved images were minimally affected by tissue morphology, endogenous absorbers, and illumination. These results demonstrate the potential of FLIM as an intraoperative diagnostic technique.

Original languageEnglish (US)
Pages (from-to)2081-2083
Number of pages3
JournalOptics Letters
Volume34
Issue number13
DOIs
StatePublished - Jul 1 2009

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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    Sun, Y., Phipps, J., Elson, D. S., Stoy, H., Tinling, S., Meier, J., Poirier, B., Chuang, F., Farwell, D. G., & Marcu, L. (2009). Fluorescence lifetime imaging microscopy: In vivo application to diagnosis of oral carcinoma. Optics Letters, 34(13), 2081-2083. https://doi.org/10.1364/OL.34.002081