Abstract
A compact clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was designed and built for intraoperative disease diagnosis and validated in vivo in a hamster oral carcinogenesis model. This apparatus allows for the remote image collection via a flexible imaging probe consisting of a gradient index objective lens and a fiber bundle. Tissue autofluorescence (337 nm excitation) was imaged using an intensified CCD with a gate width down to 0.2 ns. We demonstrate a significant contrast in fluorescence lifetime between tumor (1.77±0.26 ns) and normal (2.50±0.36 ns) tissues at 450 nm and an over 80% intensity decrease at 390 nm emission in tumor versus normal areas. The time-resolved images were minimally affected by tissue morphology, endogenous absorbers, and illumination. These results demonstrate the potential of FLIM as an intraoperative diagnostic technique.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2081-2083 |
| Number of pages | 3 |
| Journal | Optics Letters |
| Volume | 34 |
| Issue number | 13 |
| DOIs | |
| State | Published - Jul 1 2009 |
Fingerprint
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Fluorescence lifetime imaging microscopy : In vivo application to diagnosis of oral carcinoma. / Sun, Yinghua; Phipps, Jennifer; Elson, Daniel S.; Stoy, Heather; Tinling, Steven; Meier, Jeremy; Poirier, Brian; Chuang, Frank; Farwell, D Gregory; Marcu, Laura.
In: Optics Letters, Vol. 34, No. 13, 01.07.2009, p. 2081-2083.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fluorescence lifetime imaging microscopy
T2 - In vivo application to diagnosis of oral carcinoma
AU - Sun, Yinghua
AU - Phipps, Jennifer
AU - Elson, Daniel S.
AU - Stoy, Heather
AU - Tinling, Steven
AU - Meier, Jeremy
AU - Poirier, Brian
AU - Chuang, Frank
AU - Farwell, D Gregory
AU - Marcu, Laura
PY - 2009/7/1
Y1 - 2009/7/1
N2 - A compact clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was designed and built for intraoperative disease diagnosis and validated in vivo in a hamster oral carcinogenesis model. This apparatus allows for the remote image collection via a flexible imaging probe consisting of a gradient index objective lens and a fiber bundle. Tissue autofluorescence (337 nm excitation) was imaged using an intensified CCD with a gate width down to 0.2 ns. We demonstrate a significant contrast in fluorescence lifetime between tumor (1.77±0.26 ns) and normal (2.50±0.36 ns) tissues at 450 nm and an over 80% intensity decrease at 390 nm emission in tumor versus normal areas. The time-resolved images were minimally affected by tissue morphology, endogenous absorbers, and illumination. These results demonstrate the potential of FLIM as an intraoperative diagnostic technique.
AB - A compact clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was designed and built for intraoperative disease diagnosis and validated in vivo in a hamster oral carcinogenesis model. This apparatus allows for the remote image collection via a flexible imaging probe consisting of a gradient index objective lens and a fiber bundle. Tissue autofluorescence (337 nm excitation) was imaged using an intensified CCD with a gate width down to 0.2 ns. We demonstrate a significant contrast in fluorescence lifetime between tumor (1.77±0.26 ns) and normal (2.50±0.36 ns) tissues at 450 nm and an over 80% intensity decrease at 390 nm emission in tumor versus normal areas. The time-resolved images were minimally affected by tissue morphology, endogenous absorbers, and illumination. These results demonstrate the potential of FLIM as an intraoperative diagnostic technique.
UR - http://www.scopus.com/inward/record.url?scp=67650096311&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650096311&partnerID=8YFLogxK
U2 - 10.1364/OL.34.002081
DO - 10.1364/OL.34.002081
M3 - Article
C2 - 19572006
AN - SCOPUS:67650096311
VL - 34
SP - 2081
EP - 2083
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 13
ER -