Fluorescence lifetime imaging microscopy for the characterization of atherosclerotic plaques

Jennifer Phipps, Yinghua Sun, Ramez Saroufeem, Nisa Hatami, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Scopus citations

Abstract

Atherosclerotic plaque composition has been associated with plaque instability and rupture. This study investigates the use of fluorescence lifetime imaging microscopy (FLIM) for mapping plaque composition and assessing features of vulnerability. Measurements were conducted in atherosclerotic human aortic samples using an endoscopic FLIM system (spatial resolution of 35 μm; temporal resolution 200 ps) developed in our lab which allows mapping in one measurement the composition within a volume of 4 mm diameter × 250 μm depth. Each pixel in the image represents a corresponding fluorescence lifetime value; images are formed through a flexible 0.6 mm side-viewing imaging bundle which allows for further intravascular applications. Based on previously recorded spectra of human atherosclerotic plaque, fluorescence emission was collected through two filters: f 1: 377/50 and f2: 460/60 (center wavelength/bandwidth), which together provides the greatest discrimination between intrinsic fluorophores related to plaque vulnerability. We have imaged nine aortas and lifetime images were retrieved using a Laguerre expansion deconvolution technique and correlated with histopathology. Early results demonstrate discrimination using fluorescence lifetime between early, lipid-rich, and collagen-rich lesions which are consistent with previously reported time-resolved atherosclerotic plaque measurements.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7161
DOIs
StatePublished - 2009
EventPhotonic Therapeutics and Diagnostics V - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

Other

OtherPhotonic Therapeutics and Diagnostics V
CountryUnited States
CitySan Jose, CA
Period1/24/091/26/09

Keywords

  • Atherosclerotic plaque
  • Endoscopy
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Time-resolved fluorescence

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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    Phipps, J., Sun, Y., Saroufeem, R., Hatami, N., & Marcu, L. (2009). Fluorescence lifetime imaging microscopy for the characterization of atherosclerotic plaques. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7161). [71612G] https://doi.org/10.1117/12.813087