Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery

Yinghua Sun, Nisa Hatami, Matthew Yee, Jennifer Phipps, Daniel S. Elson, Fredric A Gorin, Rudolph J. Schrot, Laura Marcu

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

We demonstrate for the first time the application of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma multiforme (GBM). The clinically compatible FLIM prototype integrates a gated (down to 0.2 ns) intensifier imaging system with a fiber-bundle (fiber image guide of 0.5 mm diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, and 4 mm field of view) to provide intraoperative access to the surgical field. Experiments conducted in three patients undergoing craniotomy for tumor resection demonstrate that FLIM-derived parameters allow for delineation of tumor from normal cortex. For example, at 460±25-nm wavelength band emission corresponding to NADH/NADPH fluorescence, GBM exhibited a weaker florescence intensity (35% less, p-value <0.05) and a longer lifetime T GBM-Amean = 1.59±0.24 ns than normal cortex T NC-Amean = 1.28±0.04ns (p-value <0.005). Current results demonstrate the potential use of FLIM as a tool for image-guided surgery of brain tumors.

Original languageEnglish (US)
Article number056022
JournalJournal of Biomedical Optics
Volume15
Issue number5
DOIs
StatePublished - Sep 2010

Fingerprint

surgery
Surgery
brain
Tumors
Brain
Microscopic examination
tumors
Fluorescence
microscopy
Imaging techniques
life (durability)
fluorescence
cortexes
fibers
Fibers
intensifiers
delineation
NADP
Imaging systems
NAD

Keywords

  • Brain tumors
  • Endoscopy
  • Fluorescence lifetime imaging microscopy
  • Intraoperative cancer diagnosis
  • Malignant glioma
  • Tissue autofluorescence

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery. / Sun, Yinghua; Hatami, Nisa; Yee, Matthew; Phipps, Jennifer; Elson, Daniel S.; Gorin, Fredric A; Schrot, Rudolph J.; Marcu, Laura.

In: Journal of Biomedical Optics, Vol. 15, No. 5, 056022, 09.2010.

Research output: Contribution to journalArticle

Sun, Y, Hatami, N, Yee, M, Phipps, J, Elson, DS, Gorin, FA, Schrot, RJ & Marcu, L 2010, 'Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery', Journal of Biomedical Optics, vol. 15, no. 5, 056022. https://doi.org/10.1117/1.3486612
Sun, Yinghua ; Hatami, Nisa ; Yee, Matthew ; Phipps, Jennifer ; Elson, Daniel S. ; Gorin, Fredric A ; Schrot, Rudolph J. ; Marcu, Laura. / Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 5.
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