Fluorescence Lifetime Imaging Combined with Conventional Intravascular Ultrasound for Enhanced Assessment of Atherosclerotic Plaques: an Ex Vivo Study in Human Coronary Arteries

Hussain Fatakdawala, Dimitris Gorpas, John W Bishop, Julien Bec, Dinglong Ma, Jeffrey Southard, Kenneth B. Margulies, Laura Marcu

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

This study evaluates the ability of label-free fluorescence lifetime imaging (FLIm) to complement intravascular ultrasound (IVUS) for concurrent visualization of human coronary vessel composition, structure, and pathology. Co-registered FLIm and IVUS data from 16 coronary segments were correlated to eight distinct pathological features including thin-cap fibroatheroma (TCFA). The sensitivity, specificity, and positive predictive value for combined FLIm-IVUS (89, 99, 89 %) were better than FLIm (70, 98, 88 %) and IVUS (45, 94, 62 %) alone in distinguishing between pathologies. FLIm can assess compositional changes in luminal surface through variations in fluorescence lifetime values (<3.5 ns for lipid-rich areas; >4 ns for collagen-rich areas) enabling detection of macrophages in fibrous caps (sensitivity, 86 %) and distinguishing between relatively stable thick-cap fibroatheromas and rupture-prone TCFA (sensitivity, 80 %) amongst other features. Current results demonstrate the potential of FLIm-IVUS as a new intravascular method for improved evaluation of plaques that may subsequently aid in guiding coronary intervention.

Original languageEnglish (US)
Pages (from-to)253-263
Number of pages11
JournalJournal of Cardiovascular Translational Research
Volume8
Issue number4
DOIs
StatePublished - May 1 2015

Keywords

  • Atherosclerosis
  • Cardiovascular diseases
  • Fluorescence lifetime imaging
  • Intravascular imaging
  • Intravascular ultrasound

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Genetics
  • Genetics(clinical)
  • Molecular Medicine
  • Pharmaceutical Science

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