Fluorescence enhancement and focal volume reduction observed in c-shaped nano-apertures

Yin Yuen, Samantha Fore, Thomas R Huser, Lambertus Hesselink

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We evaluate the potential ability of c-shaped apertures milled in aluminum thin films to reduce the effective measurement volume and to enhance the fluorescence signal for fluorescence correlation spectroscopy of ATT0655 dye dissolved in a HEPES buffer solution. Previous studies have shown that by morphing a square aperture into a rectangular aperture while holding the cross-sectional area constant will yield strong polarization dependence in the reduction of the effective volume and about a factor of 2-3 enhancement in the fluorescence count rate per molecule. By morphing the rectangular aperture into a c-shaped aperture we gain further reduction in focal volume while maintaining the count rate enhancements. In particular, we compare c-shaped apertures to squares with the same cross-sectional area and show that one can achieve one molecule per focal volume at ∼3μM (about a 1000 times reduction in effective volume compared to confocal FCS) while maintaining a fluorescence count rate per molecule of about an order of magnitude higher than for bulk diffusing dyes. Two orthogonal polarizations for the incident field have been studied to explore the effects on the focal volume reduction and fluorescence count rate enhancements.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6869
DOIs
StatePublished - 2008
EventPlasmonics in Biology and Medicine V - San Jose, CA, United States
Duration: Jan 21 2008Jan 22 2008

Other

OtherPlasmonics in Biology and Medicine V
CountryUnited States
CitySan Jose, CA
Period1/21/081/22/08

Keywords

  • c-aperture
  • FCS
  • Fluorescence
  • Focal volume reduction
  • Metal enhanced fluorescence
  • Nano-aperture
  • Nano-structured surface
  • Resonance

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Yuen, Y., Fore, S., Huser, T. R., & Hesselink, L. (2008). Fluorescence enhancement and focal volume reduction observed in c-shaped nano-apertures. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6869). [68690U] https://doi.org/10.1117/12.764053