Fluorescein-conjugated Bandeiraea simplicifolia lectin as a marker of endodermal, Yolk Sac, and trophoblastic differentiation in the mouse embryo

T. C. Wu, Yu-Jui Yvonne Wan, I. Damjanov

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The Bandeiraea simplicifolia lectin I (BSA-I) conjugated to fluorescein isothiocyanate was used as a histochemical reagent to study the mouse embryos from fertilization to early somitogenesis. No lectin binding could be detected on the embryonic cells in the preimplantation embryo. Lectin labeled intensely the zona pellucida. In the implanting embryos lectin binding was detected along the subtrophectodermal and Reichert's membrane, in the cytoplasm of the parietal and visceral endoderm, and the trophoblastic giant cells, but not in the ectodermal cells. Studies on explanted blastocysts cultured in vitro disclosed that the cytoplasmic BSA-I binding sites in trophoblastic cells develop gradually. In the 9-day somitic embryo BSA-I reacted with epithelial cells of the yolk sac, but not with the mesenchymal cells. A continuity between the lectin-reactive endoderm and the foregut epithelium could be demonstrated. These data indicated that BSA-I lectin can be used as a histochemical probe for endodermal (yolk sac) and trophoblastic differentiation in the peri-implantational mouse embryo.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalDifferentiation
Volume24
Issue number1
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Pathology and Forensic Medicine

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