Abstract
We measured the rate of fluid movement across the tracheal epithelium of the dog by an electrical method, modified from that originally described by Wiedner (Rev. Sci. Instrum. 47: 775-6, 1976). These base-line volume flow (J(v) from the submucosa to the mucosa was 44 ± 23 nl·min-1·cm-2 (mean ± SE; n = 6). In only three of the tissues was the resting J(v) significantly greater than zero. Stimulation with 2 x 10-3 M aminophylline (a drug that we have shown stimulates Cl secretion under short-circuit conditions) increased J(v) in all tissues to a mean of 137 ± 29 nl·min-1·cm-2 (P < 0.001). We also measured the unidirectional fluxes of Cl with the tissue at its spontaneous electrical potential. During the base-line period, the net flux of Cl was not significantly different from zero. During stimulation with aminophylline, Cl secretion increased to a mean of 1.44 ± 0.50 μeq·cm-2·h-1. Comparison of the stimulated values of J(v) and net Cl secretion suggest that fluid secretion by the trachea is isotonic during stimulation with aminophylline.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 905-909 |
| Number of pages | 5 |
| Journal | Journal of Applied Physiology Respiratory Environmental and Exercise Physiology |
| Volume | 49 |
| Issue number | 5 |
| State | Published - 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Physiology
Cite this
Fluid transport across the canine tracheal epithelium. / Welsh, M. J.; Widdicombe, Jonathan; Nadel, J. A.
In: Journal of Applied Physiology Respiratory Environmental and Exercise Physiology, Vol. 49, No. 5, 1980, p. 905-909.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fluid transport across the canine tracheal epithelium
AU - Welsh, M. J.
AU - Widdicombe, Jonathan
AU - Nadel, J. A.
PY - 1980
Y1 - 1980
N2 - We measured the rate of fluid movement across the tracheal epithelium of the dog by an electrical method, modified from that originally described by Wiedner (Rev. Sci. Instrum. 47: 775-6, 1976). These base-line volume flow (J(v) from the submucosa to the mucosa was 44 ± 23 nl·min-1·cm-2 (mean ± SE; n = 6). In only three of the tissues was the resting J(v) significantly greater than zero. Stimulation with 2 x 10-3 M aminophylline (a drug that we have shown stimulates Cl secretion under short-circuit conditions) increased J(v) in all tissues to a mean of 137 ± 29 nl·min-1·cm-2 (P < 0.001). We also measured the unidirectional fluxes of Cl with the tissue at its spontaneous electrical potential. During the base-line period, the net flux of Cl was not significantly different from zero. During stimulation with aminophylline, Cl secretion increased to a mean of 1.44 ± 0.50 μeq·cm-2·h-1. Comparison of the stimulated values of J(v) and net Cl secretion suggest that fluid secretion by the trachea is isotonic during stimulation with aminophylline.
AB - We measured the rate of fluid movement across the tracheal epithelium of the dog by an electrical method, modified from that originally described by Wiedner (Rev. Sci. Instrum. 47: 775-6, 1976). These base-line volume flow (J(v) from the submucosa to the mucosa was 44 ± 23 nl·min-1·cm-2 (mean ± SE; n = 6). In only three of the tissues was the resting J(v) significantly greater than zero. Stimulation with 2 x 10-3 M aminophylline (a drug that we have shown stimulates Cl secretion under short-circuit conditions) increased J(v) in all tissues to a mean of 137 ± 29 nl·min-1·cm-2 (P < 0.001). We also measured the unidirectional fluxes of Cl with the tissue at its spontaneous electrical potential. During the base-line period, the net flux of Cl was not significantly different from zero. During stimulation with aminophylline, Cl secretion increased to a mean of 1.44 ± 0.50 μeq·cm-2·h-1. Comparison of the stimulated values of J(v) and net Cl secretion suggest that fluid secretion by the trachea is isotonic during stimulation with aminophylline.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019151162&partnerID=8YFLogxK
M3 - Article
C2 - 7429914
AN - SCOPUS:0019151162
VL - 49
SP - 905
EP - 909
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 5
ER -