Fluid percussion injury device for the precise control of injury parameters

Radia Abdul Wahab, Eric J. Neuberger, Bruce G Lyeth, Vijayalakshmi Santhakumar, Bryan J. Pfister

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Injury to the brain can occur from a variety of physical insults and the degree of disability can greatly vary from person to person. It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as magnitude, direction and speed of the forces acting on the head. New method: To model variations in the biomechanical injury parameters, a voice coil driven fluid percussion injury (FPI) system was designed and built to generate fluid percussion waveforms with adjustable rise times, peak pressures, and durations. Using this system, pathophysiological outcomes in the rat were investigated and compared to animals injured with the same biomechanical parameters using the pendulum based FPI system. Results in comparison with existing methods: Immediate post-injury behavior shows similar rates of seizures and mortality in adolescent rats and similar righting times, toe pinch responses and mortality rates in adult rats. Interestingly, post injury mortality in adult rats was sensitive to changes in injury rate. Fluoro-Jade labeling of degenerating neurons in the hilus and CA2-3 hippocampus were consistent between injuries produced with the voice coil and pendulum operated systems. Granule cell population spike amplitude to afferent activation, a measure of dentate network excitability, also showed consistent enhancement 1 week after injury using either system. Conclusions: Overall our results suggest that this new FPI device produces injury outcomes consistent with the commonly used pendulum FPI system and has the added capability to investigate pathophysiology associated with varying rates and durations of injury.

Original languageEnglish (US)
Pages (from-to)16-26
Number of pages11
JournalJournal of Neuroscience Methods
Volume248
DOIs
StatePublished - Jun 5 2015

Fingerprint

Percussion
Equipment and Supplies
Wounds and Injuries
Mortality
Toes
Brain Injuries

Keywords

  • Brain injury
  • Fluid percussion injury
  • Injury biomechanics
  • Injury rate

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fluid percussion injury device for the precise control of injury parameters. / Wahab, Radia Abdul; Neuberger, Eric J.; Lyeth, Bruce G; Santhakumar, Vijayalakshmi; Pfister, Bryan J.

In: Journal of Neuroscience Methods, Vol. 248, 05.06.2015, p. 16-26.

Research output: Contribution to journalArticle

Wahab, Radia Abdul ; Neuberger, Eric J. ; Lyeth, Bruce G ; Santhakumar, Vijayalakshmi ; Pfister, Bryan J. / Fluid percussion injury device for the precise control of injury parameters. In: Journal of Neuroscience Methods. 2015 ; Vol. 248. pp. 16-26.
@article{306d15c0432d4310be541f69e0ebcb1d,
title = "Fluid percussion injury device for the precise control of injury parameters",
abstract = "Background: Injury to the brain can occur from a variety of physical insults and the degree of disability can greatly vary from person to person. It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as magnitude, direction and speed of the forces acting on the head. New method: To model variations in the biomechanical injury parameters, a voice coil driven fluid percussion injury (FPI) system was designed and built to generate fluid percussion waveforms with adjustable rise times, peak pressures, and durations. Using this system, pathophysiological outcomes in the rat were investigated and compared to animals injured with the same biomechanical parameters using the pendulum based FPI system. Results in comparison with existing methods: Immediate post-injury behavior shows similar rates of seizures and mortality in adolescent rats and similar righting times, toe pinch responses and mortality rates in adult rats. Interestingly, post injury mortality in adult rats was sensitive to changes in injury rate. Fluoro-Jade labeling of degenerating neurons in the hilus and CA2-3 hippocampus were consistent between injuries produced with the voice coil and pendulum operated systems. Granule cell population spike amplitude to afferent activation, a measure of dentate network excitability, also showed consistent enhancement 1 week after injury using either system. Conclusions: Overall our results suggest that this new FPI device produces injury outcomes consistent with the commonly used pendulum FPI system and has the added capability to investigate pathophysiology associated with varying rates and durations of injury.",
keywords = "Brain injury, Fluid percussion injury, Injury biomechanics, Injury rate",
author = "Wahab, {Radia Abdul} and Neuberger, {Eric J.} and Lyeth, {Bruce G} and Vijayalakshmi Santhakumar and Pfister, {Bryan J.}",
year = "2015",
month = "6",
day = "5",
doi = "10.1016/j.jneumeth.2015.03.010",
language = "English (US)",
volume = "248",
pages = "16--26",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",

}

TY - JOUR

T1 - Fluid percussion injury device for the precise control of injury parameters

AU - Wahab, Radia Abdul

AU - Neuberger, Eric J.

AU - Lyeth, Bruce G

AU - Santhakumar, Vijayalakshmi

AU - Pfister, Bryan J.

PY - 2015/6/5

Y1 - 2015/6/5

N2 - Background: Injury to the brain can occur from a variety of physical insults and the degree of disability can greatly vary from person to person. It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as magnitude, direction and speed of the forces acting on the head. New method: To model variations in the biomechanical injury parameters, a voice coil driven fluid percussion injury (FPI) system was designed and built to generate fluid percussion waveforms with adjustable rise times, peak pressures, and durations. Using this system, pathophysiological outcomes in the rat were investigated and compared to animals injured with the same biomechanical parameters using the pendulum based FPI system. Results in comparison with existing methods: Immediate post-injury behavior shows similar rates of seizures and mortality in adolescent rats and similar righting times, toe pinch responses and mortality rates in adult rats. Interestingly, post injury mortality in adult rats was sensitive to changes in injury rate. Fluoro-Jade labeling of degenerating neurons in the hilus and CA2-3 hippocampus were consistent between injuries produced with the voice coil and pendulum operated systems. Granule cell population spike amplitude to afferent activation, a measure of dentate network excitability, also showed consistent enhancement 1 week after injury using either system. Conclusions: Overall our results suggest that this new FPI device produces injury outcomes consistent with the commonly used pendulum FPI system and has the added capability to investigate pathophysiology associated with varying rates and durations of injury.

AB - Background: Injury to the brain can occur from a variety of physical insults and the degree of disability can greatly vary from person to person. It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as magnitude, direction and speed of the forces acting on the head. New method: To model variations in the biomechanical injury parameters, a voice coil driven fluid percussion injury (FPI) system was designed and built to generate fluid percussion waveforms with adjustable rise times, peak pressures, and durations. Using this system, pathophysiological outcomes in the rat were investigated and compared to animals injured with the same biomechanical parameters using the pendulum based FPI system. Results in comparison with existing methods: Immediate post-injury behavior shows similar rates of seizures and mortality in adolescent rats and similar righting times, toe pinch responses and mortality rates in adult rats. Interestingly, post injury mortality in adult rats was sensitive to changes in injury rate. Fluoro-Jade labeling of degenerating neurons in the hilus and CA2-3 hippocampus were consistent between injuries produced with the voice coil and pendulum operated systems. Granule cell population spike amplitude to afferent activation, a measure of dentate network excitability, also showed consistent enhancement 1 week after injury using either system. Conclusions: Overall our results suggest that this new FPI device produces injury outcomes consistent with the commonly used pendulum FPI system and has the added capability to investigate pathophysiology associated with varying rates and durations of injury.

KW - Brain injury

KW - Fluid percussion injury

KW - Injury biomechanics

KW - Injury rate

UR - http://www.scopus.com/inward/record.url?scp=84927625394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927625394&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2015.03.010

DO - 10.1016/j.jneumeth.2015.03.010

M3 - Article

VL - 248

SP - 16

EP - 26

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

ER -