Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model

G. B. Nackman, M. F. Fillinger, R. Shafritz, T. Wei, A. M. Graham, J. A. Freischlag, K. C. Kent

Research output: Contribution to journalArticle

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Abstract

Background. With a co-culture model, we have previously demonstrated that endothelial cells (ECs) exert regulatory control over smooth muscle cell (SMC) behavior. ECs appeared to stimulate SMC proliferation in static culture. This Study was performed to test the hypothesis that the EC stimulation of SMC proliferation was effected by shear stress. Methods. Bovine SMCs were cultured on a thin semipermeable membrane either alone or opposite ECs in co-culture (SMC/EC). A novel parallel-plate flow device was developed and used for exposing the EC side of the co-culture to shear stress. EC and SMC proliferation rates were determined after 24 hours' exposure to 0, 1, or 10 dynes/cm2 of shear stress. Results. SMC proliferation decreased significantly from 362 ± 65 cpm/μg DNA (control, mean ± SEM) to 68 ± 43 cpm/μg (1 dyne/cm2) and 99 ± 18 cpm/μg (10 dynes/cm2) (P <. 05). EC proliferation after flow decreased as compared with no-flow controls 71 ± 15 cpm/μg DNA (control, mean ± SEM) to 29 ± 5 cpm/μg (1 dyne/cm2) and 21 ± 4 cpm/μg (10 dynes/cm2) (P < .05). Conclusions. In a model designed to study SMC/EC interactions in a flow environment, it was seen that EC exposure to shear stress alters the growth characteristics of SMCs. This suggests that hemodynamic mechanical forces may be sufficient to alter the EC regulation of SMC behavior.

Original languageEnglish (US)
Pages (from-to)353-361
Number of pages9
JournalSurgery
Volume124
Issue number2
DOIs
StatePublished - 1998
Externally publishedYes

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Smooth Muscle Myocytes
Endothelial Cells
Cell Proliferation
Coculture Techniques
DNA
Cell Communication
Hemodynamics
Equipment and Supplies
Membranes
Growth

ASJC Scopus subject areas

  • Surgery

Cite this

Nackman, G. B., Fillinger, M. F., Shafritz, R., Wei, T., Graham, A. M., Freischlag, J. A., & Kent, K. C. (1998). Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model. Surgery, 124(2), 353-361. https://doi.org/10.1016/S0039-6060(98)70141-2

Flow modulates endothelial regulation of smooth muscle cell proliferation : A new model. / Nackman, G. B.; Fillinger, M. F.; Shafritz, R.; Wei, T.; Graham, A. M.; Freischlag, J. A.; Kent, K. C.

In: Surgery, Vol. 124, No. 2, 1998, p. 353-361.

Research output: Contribution to journalArticle

Nackman, GB, Fillinger, MF, Shafritz, R, Wei, T, Graham, AM, Freischlag, JA & Kent, KC 1998, 'Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model', Surgery, vol. 124, no. 2, pp. 353-361. https://doi.org/10.1016/S0039-6060(98)70141-2
Nackman GB, Fillinger MF, Shafritz R, Wei T, Graham AM, Freischlag JA et al. Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model. Surgery. 1998;124(2):353-361. https://doi.org/10.1016/S0039-6060(98)70141-2
Nackman, G. B. ; Fillinger, M. F. ; Shafritz, R. ; Wei, T. ; Graham, A. M. ; Freischlag, J. A. ; Kent, K. C. / Flow modulates endothelial regulation of smooth muscle cell proliferation : A new model. In: Surgery. 1998 ; Vol. 124, No. 2. pp. 353-361.
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