Flow cytometry of fine needle aspirations of the Sprague-Dawley rat testis

defining normal maturation and the effects of multiple biopsies.

S. J. Skoog, Christopher P Evans, I. J. Hayward, J. L. Griffin, C. L. Hitchcock

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Quantitative assessment of spermatogenesis by flow cytometry reproducibly correlates with histological examination. Flow cytometry of fine needle aspirates from the testis was used to analyze normal spermatogenesis of Sprague-Dawley rats. Aged matched male weanlings were divided into 3 groups: group 1-5 rats underwent bilateral percutaneous aspiration of the testes with a 22 gauge Chiba needle on postpartum days 19, 24, 30 and 40; group 2-5 rats aspirated on days 24, 30 and 40, and group 3-4 rats aspirated on days 30 and 40. This sequential approach allowed for evaluation of normal spermatogenesis and the effects of repeated fine needle aspiration on spermatogenesis. Deoxyribonucleic acid distribution analysis by flow cytometry correlates with haploid (1N), diploid (2N), tetraploid (4N) and S phase cell populations. These cell populations were evaluated and comparisons among each group were made at all biopsy times. No significant differences in mean 2N, 4N or S phase cell populations in single versus repeat biopsied testes were detected. However, there was a significant increase in the 1N population at 30 days post partum in the repeat biopsy group (p = 0.037), which normalized by day 40. This increase in 1N corresponds to the beginning of meiosis, which is maximal between postpartum days 30 and 40, and occurs earlier in the repeat biopsied testis. Repeat fine needle aspiration of the testis can be performed without significantly affecting spermatogenesis in the "weanling" rat. This provides a useful technique in the future investigations of the time related effects of varicocele, chemotherapy and toxicologic drugs on spermatogenesis.

Original languageEnglish (US)
JournalJournal of Urology
Volume146
Issue number2
StatePublished - Aug 1991
Externally publishedYes

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Spermatogenesis
Fine Needle Biopsy
Sprague Dawley Rats
Testis
Flow Cytometry
Biopsy
S Phase
Postpartum Period
Population
Needles
Varicocele
Tetraploidy
Haploidy
Meiosis
Diploidy
Drug Therapy
DNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Urology

Cite this

Flow cytometry of fine needle aspirations of the Sprague-Dawley rat testis : defining normal maturation and the effects of multiple biopsies. / Skoog, S. J.; Evans, Christopher P; Hayward, I. J.; Griffin, J. L.; Hitchcock, C. L.

In: Journal of Urology, Vol. 146, No. 2, 08.1991.

Research output: Contribution to journalArticle

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